Universität zu Köln

Rinklin, Philipp, Afanasenkau, Dzmitry, Wiegand, Simone ORCID: 0000-0001-6333-1956, Offenhaeusser, Andreas and Wolfrum, Bernhard ORCID: 0000-0003-4438-3755 (2015). Inducing microscopic thermal lesions for the dissection of functional cell networks on a chip. Lab Chip, 15 (1). S. 237 - 244. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 1473-0189

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Abstract

We present a versatile chip-based method to inflict microscopic lesions on cellular networks or tissue models. Our approach relies on resistive heating of microstructured conductors to impose highly localized thermal stress on specific regions of a cell network. We show that networks can be precisely dissected into individual subnetworks using a microwire crossbar array. To this end, we pattern a network of actively beating cardiomyocyte-like cells into smaller subunits by inflicting thermal damage along selected wires of the array. We then investigate the activity and functional connectivity of the individual subnetworks using a Ca2+ imaging-based signal propagation analysis. Our results demonstrate the efficient separation of functional activity between individual subnetworks on a microscopic level. We believe that the presented technique may become a powerful tool for investigating lesion and regeneration models in cellular networks.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rinklin, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Afanasenkau, Dzmitry UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiegand, Simone UNSPECIFIED orcid.org/0000-0001-6333-1956 UNSPECIFIED Offenhaeusser, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolfrum, Bernhard UNSPECIFIED orcid.org/0000-0003-4438-3755 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-419288
DOI:	10.1039/c4lc00805g
Journal or Publication Title:	Lab Chip
Volume:	15
Number:	1
Page Range:	S. 237 - 244
Date:	2015
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	1473-0189
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO MODEL; MICROFLUIDIC CULTURE PLATFORM; MICROELECTRODE ARRAY; SPINAL-CORD; NEURITE OUTGROWTH; HL-1 CELLS; NEUROSCIENCE; REGENERATION; INHIBITORS; DENSITY Multiple languages Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology; Instruments & Instrumentation Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/41928