Universität zu Köln

Blankenburg, S., Balfanz, S., Hayashi, Y., Shigenobu, S., Miura, T., Baumann, O., Baumann, A. and Blenau, W. (2015). Cockroach GABA(B) receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution. Neuropharmacology, 88. S. 134 - 145. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-7064

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Abstract

gamma-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABA(A) receptors or metabotropic GABA(B) receptors. GABA(B) receptors regulate, via Gi/o, G-proteins, ion channels, and adenylyl cyclases. In humans, GABA(B) receptor subtypes are involved in the etiology of neurologic and psychiatric disorders. In arthropods, however, these members of the G-protein-coupled receptor family are only inadequately characterized. Interestingly, physiological data have revealed important functions of GABA(B) receptors in the American cockroach, Periplaneta americana. We have cloned cDNAs coding for putative GABA(B) receptor subtypes 1 and 2 of P. americana (PeaGB1 and PeaGB2). When both receptor proteins are co-expressed in mammalian cells, activation of the receptor heteromer with GABA leads to a dose-dependent decrease in cAMP production. The pharmacological profile differs from that of mammalian and Drosophila GABA(B) receptors. Western blot analyses with polyclonal antibodies have revealed the expression of PeaGB1 and PeaGB2 in the CNS of the American cockroach. In addition to the widespread distribution in the brain, PeaGB1 is expressed in salivary glands and male accessory glands. Notably, PeaGB1-like immunoreactivity has been detected in the GABAergic salivary neuron 2, suggesting that GABA(B) receptors act as autoreceptors in this neuron. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Blankenburg, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Balfanz, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hayashi, Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shigenobu, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Miura, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Blenau, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-419838
DOI:	10.1016/j.neuropharm.2014.08.022
Journal or Publication Title:	Neuropharmacology
Volume:	88
Page Range:	S. 134 - 145
Date:	2015
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-7064
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMINOBUTYRIC-ACID RECEPTOR; PERIPLANETA-AMERICANA; IMMUNOCYTOCHEMICAL LOCALIZATION; IMMUNOREACTIVE NEURONS; SUBESOPHAGEAL GANGLION; SYNAPTIC CONNECTIONS; EXTRACELLULAR DOMAIN; INTERNATIONAL UNION; MUSHROOM BODIES; CENTRAL COMPLEX Multiple languages Neurosciences; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41983