Universität zu Köln

Guenal-Sadik, Gamze, Paszkowski-Rogacz, Maciej, Singaravelu, Kalaimathy, Beyer, Andreas ORCID: 0000-0002-3891-2123, Buchholz, Frank ORCID: 0000-0002-4577-3344 and Jessberger, Rolf (2014). Stage-Specific Binding Profiles of Cohesin in Resting and Activated B Lymphocytes Suggest a Role for Cohesin in Immunoglobulin Class Switching and Maturation. PLoS One, 9 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The immunoglobulin heavy chain locus (Igh) features higher-order chromosomal interactions to facilitate stage-specific assembly of the Ig molecule. Cohesin, a ring-like protein complex required for sister chromatid cohesion, shapes chromosome architecture and chromatin interactions important for transcriptional regulation and often acts together with CTCF. Cohesin is likely involved in B cell activation and Ig class switch recombination. Hence, binding profiles of cohesin in resting mature murine splenic B lymphocytes and at two stages after cell activation were elucidated by chromatin immunoprecipitation and deep sequencing. Comparative genomic analysis revealed cohesin extensively changes its binding to transcriptional control elements after 48 h of stimulation with LPS/IL-4. Cohesin was clearly underrepresented at switch regions regardless of their activation status, suggesting that switch regions need to be cohesin-poor. Specific binding changes of cohesin at B-cell specific gene loci Pax5 and Blimp-1 indicate new cohesin-dependent regulatory pathways. Together with conserved cohesin/CTCF sites at the Igh 3'RR, a prominent cohesin/CTCF binding site was revealed near the 3' end of C alpha where PolII localizes to 3' enhancers. Our study shows that cohesin likely regulates B cell activation and maturation, including Ig class switching.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Guenal-Sadik, Gamze UNSPECIFIED UNSPECIFIED UNSPECIFIED Paszkowski-Rogacz, Maciej UNSPECIFIED UNSPECIFIED UNSPECIFIED Singaravelu, Kalaimathy UNSPECIFIED UNSPECIFIED UNSPECIFIED Beyer, Andreas UNSPECIFIED orcid.org/0000-0002-3891-2123 UNSPECIFIED Buchholz, Frank UNSPECIFIED orcid.org/0000-0002-4577-3344 UNSPECIFIED Jessberger, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-423550
DOI:	10.1371/journal.pone.0111748
Journal or Publication Title:	PLoS One
Volume:	9
Number:	11
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 3' REGULATORY REGION; PLASMA-CELL DIFFERENTIATION; GENE-EXPRESSION; IGH LOCUS; V(D)J RECOMBINATION; CTCF; ARCHITECTURE; ELEMENTS; IDENTIFICATION; REARRANGEMENT Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42355