Universität zu Köln

de la Torre, Xavier ORCID: 0000-0001-8037-6750, Colamonici, Cristiana, Curcio, Davide, Jardines, Daniel, Molaioni, Francesco, Parr, Maria Kristina ORCID: 0000-0001-7407-8300 and Botre, Francesco (2014). Detection of formestane abuse by mass spectrometric techniques. Drug Test. Anal., 6 (11-12). S. 1133 - 1141. HOBOKEN: WILEY-BLACKWELL. ISSN 1942-7611

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Abstract

Formestane (4-hydroxy-androstenedione) is an aromatase inhibitor prohibited in sports and included, since 2004, in the list of prohibited substances updated yearly by the World Anti-Doping Agency (WADA). Since the endogenous production of formestane has been described, it is mandatory for the anti-doping laboratories to use isotope ratio mass spectrometry (IRMS) to establish the exogenous origin before issuing an adverse analytical finding. The described IRMS methods for formestane detection are time-consuming, requiring usually two consecutive liquid chromatographic sample purifications in order to have final extracts of adequate purity before the mass spectrometric analysis. After establishing a procedure for the determination of the origin of formestane by IRMS without the need of derivatization, and integrated in the overall analytical strategy of the laboratory for pseudo-endogenous steroids, a mass spectrometric analysis by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) of formestane metabolites was carried out in order to investigate whether other biomarkers of formestane abuse could be integrated in order to avoid time-consuming and expensive IRMS confirmations for formestane. From the metabolic studies performed, the inclusion of 3,4-dihydroxy-5-androstan-17-one (4-hydroxy-epiandosterone) in the routine GC-MS procedures has demonstrated to be diagnostic in order to reduce the number of unnecessary confirmations of the endogenous origin of formestane. Copyright (c) 2014 John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code de la Torre, Xavier UNSPECIFIED orcid.org/0000-0001-8037-6750 UNSPECIFIED Colamonici, Cristiana UNSPECIFIED UNSPECIFIED UNSPECIFIED Curcio, Davide UNSPECIFIED UNSPECIFIED UNSPECIFIED Jardines, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Molaioni, Francesco UNSPECIFIED UNSPECIFIED UNSPECIFIED Parr, Maria Kristina UNSPECIFIED orcid.org/0000-0001-7407-8300 UNSPECIFIED Botre, Francesco UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-423955
DOI:	10.1002/dta.1759
Journal or Publication Title:	Drug Test. Anal.
Volume:	6
Number:	11-12
Page Range:	S. 1133 - 1141
Date:	2014
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BREAST-CANCER PATIENTS; METABOLISM; 4-HYDROXYANDROSTENEDIONE; IDENTIFICATION Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42395