Universität zu Köln

Reddy, Lavakumar A., Mikesh, Leann, Moskulak, Christopher, Harvey, Jennifer, Sherman, Nicholas ORCID: 0000-0003-3206-3113, Zigrino, Paola ORCID: 0000-0002-7470-0064, Mauch, Cornelia and Fox, Jay W. (2014). Host Response to Human Breast Invasive Ductal Carcinoma (IDC) as Observed by Changes in the Stromal Proteome. J. Proteome Res., 13 (11). S. 4739 - 4752. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

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Abstract

Following initial transformation, tumorigenesis, growth, invasion, and metastasis involves a complex interaction between the transformed tissue and the host, particularly in the microenvironment adjacent to the developing tumor. The tumor microenvironment itself is a unique outcome of the host reacting to the tumor and perhaps the tumor reacting to the host and in turn the tumor altering the host's response to give rise to an environment that ultimately promotes tumor progression. The tumor-adjacent stromal, sometimes referred to as reactive stromal or the desmoplastic stroma, has received some investigative studies, but it is incomplete, and likely different tumors promote a varied response and hence different reactive stroma. In this study, we have investigated the proteomics of the host response, both in vitro and in vivo, to breast epithelial cancer, in the former using tissue culture and in the latter laser microdissection of stromal tissue both adjacent and distal to breast invasive ductal cancer (IDC). From proteomic analysis of in vitro tissue culture studies, we observed that the stroma produced is related to the invasiveness of the stimulating breast cancer cell lines but different from that observed from the stromal proteome of archival tissue. In vivo we have identified several potential markers of a reactive stroma. Furthermore, we observed that the proteome of tumor-adjacent stroma differs from that of tumor-distal stroma. The proteomic description of human breast IDC stroma may serve to enhance our understanding of the role of stroma in the progression of cancer and may suggest potential mechanisms of therapeutic interdiction.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reddy, Lavakumar A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mikesh, Leann UNSPECIFIED UNSPECIFIED UNSPECIFIED Moskulak, Christopher UNSPECIFIED UNSPECIFIED UNSPECIFIED Harvey, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Sherman, Nicholas UNSPECIFIED orcid.org/0000-0003-3206-3113 UNSPECIFIED Zigrino, Paola UNSPECIFIED orcid.org/0000-0002-7470-0064 UNSPECIFIED Mauch, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Fox, Jay W. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-424524
DOI:	10.1021/pr500620x
Journal or Publication Title:	J. Proteome Res.
Volume:	13
Number:	11
Page Range:	S. 4739 - 4752
Date:	2014
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX; CANCER PROGRESSION; COLLAGEN FIBRILS; PERIOSTIN; EXPRESSION; CELL; XII; SIGNATURES; CLEAVAGE; CLONING Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42452