Universität zu Köln

Hucklenbroich, Joerg, Klein, Rebecca, Neumaier, Bernd ORCID: 0000-0001-5425-3116, Graf, Rudolf, Fink, Gereon Rudolf, Schroeter, Michael and Rueger, Maria Adele (2014). Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo. Stem Cell Res. Ther., 5. LONDON: BMC. ISSN 1757-6512

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Abstract

Introduction: Aromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated. Methods: We exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naive rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [F-18]-fluoro-L-thymidine ([F-18] FLT), as well as ex vivo. Results: In vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [F-18] FLT-PET and histology (P < 0.05). Conclusions: Both in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hucklenbroich, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Rebecca UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumaier, Bernd UNSPECIFIED orcid.org/0000-0001-5425-3116 UNSPECIFIED Graf, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Gereon Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueger, Maria Adele UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-428442
DOI:	10.1186/scrt500
Journal or Publication Title:	Stem Cell Res. Ther.
Volume:	5
Date:	2014
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1757-6512
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language KAPPA-B; NEUROGENESIS; PROGENITORS; ACTIVATION; NEUROINFLAMMATION; ISCHEMIA; DISEASE; PROTEIN; INJURY Multiple languages Cell & Tissue Engineering; Cell Biology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42844