Universität zu Köln

Shimabukuro-Vornhagen, Alexander ORCID: 0000-0002-2351-7294, Schloesser, Hans A., Gryschok, Luise, Malcher, Joke, Wennhold, Kerstin ORCID: 0000-0001-5539-7226, Garcia-Marquez, Maria, Herbold, Till, Neuhaus, Laura S., Becker, Hans J., Fiedler, Anne, Scherwitz, Pascal, Koslowsky, Thomas, Hake, Roland, Stippel, Dirk L., Hoelscher, Arnulf H., Eidt, Sebastian, Hallek, Michael, Theurich, Sebastian ORCID: 0000-0001-5706-8258 and von Bergwelt-Baildon, Michael S. (2014). Characterization of tumor-associated B-cell subsets in patients with colorectal cancer. Oncotarget, 5 (13). S. 4651 - 4665. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

Purpose: A precise understanding of the mechanisms by which human immune cell subsets affect tumor biology will be critical for successful treatment of cancer using immunotherapeutic approaches. Recent evidence suggests that B cells can both promote and inhibit the development and progression of tumors. The aim of this study was to characterize the composition of the B-cell infiltrates in colorectal cancers (CRC) in order to gain further insight into the role of B cells in CRC. Experimental Design: In this study we characterized B-cell subsets in primary tumors (n=38), metastases (n=6) and blood (n=46) of 51 patients with a diagnosis of CRC and blood of 10 healthy controls. B-cell subsets were analyzed by flow cytometry or immunohistochemistry. Results: Peripheral blood of CRC patients contained a higher percentage of memory B cells than that of age-matched healthy controls. Furthermore, the percentage of B cells within tumors was higher than that in the peripheral blood of CRC patients while metastases were typically devoid of tumor-infiltrating B cells. Tumor-associated B cells were enriched for activated and terminally differentiated B cells. Relevant proportions of regulatory B cells could only be detected in advanced cancer and metastases. Conclusion: B cells constitute a significant proportion of the immune infiltrate in CRC. The B-cell infiltrate of primary CRC is characterized by an accumulation of terminally differentiated memory B cells or plasma cells suggestive of a specific immune response against the tumor. However advanced tumors and metastases are also infiltrated by a considerable number of regulatory B cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shimabukuro-Vornhagen, Alexander UNSPECIFIED orcid.org/0000-0002-2351-7294 UNSPECIFIED Schloesser, Hans A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gryschok, Luise UNSPECIFIED UNSPECIFIED UNSPECIFIED Malcher, Joke UNSPECIFIED UNSPECIFIED UNSPECIFIED Wennhold, Kerstin UNSPECIFIED orcid.org/0000-0001-5539-7226 UNSPECIFIED Garcia-Marquez, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Herbold, Till UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuhaus, Laura S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Hans J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fiedler, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherwitz, Pascal UNSPECIFIED UNSPECIFIED UNSPECIFIED Koslowsky, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hake, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Stippel, Dirk L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelscher, Arnulf H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eidt, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallek, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Theurich, Sebastian UNSPECIFIED orcid.org/0000-0001-5706-8258 UNSPECIFIED von Bergwelt-Baildon, Michael S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433833
DOI:	10.18632/oncotarget.1701
Journal or Publication Title:	Oncotarget
Volume:	5
Number:	13
Page Range:	S. 4651 - 4665
Date:	2014
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1949-2553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SOLID TUMORS; INFILTRATING LYMPHOCYTES; BREAST-CANCER; IMMUNE CELLS; FAVORABLE PROGNOSIS; ANTITUMOR IMMUNITY; REGULATORY CELLS; T-CELLS; MELANOMA; ANTIGEN Multiple languages Oncology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43383