Universität zu Köln

Sturm, Sebastian and Predel, Reinhard (2014). Serine phosphorylation of CAPA pyrokinin in cockroaches-A taxon-specific posttranslational modification. Peptides, 57. S. 52 - 59. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1873-5169

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Abstract

In insects, posttranslational modifications of neuropeptides are largely restricted to C- and N-terminal amino acids. The most common modifications, N-terminal pyroglutamate formation and C-terminal alpha-amidation, may prevent a fast degradation of these messenger molecules. This is particularly important for peptide hormones. Other common posttranslational modifications of proteins such as glycosylation and phosphorylation seem to be very rare in insect neuropeptides. To check this assumption, we used a computer algorithm to search an extensive data set of MALDI-TOF mass spectra from cockroach tissues for ion signal patterns indicating peptide phosphorylation. The results verify that phosphorylation is indeed very rare. However, a candidate was found and experimentally verified as phosphorylated CAPA pyrokinin (GGGGpSGETSGMWFGPRL-NH2) in the cockroach Lamproblatta albipalpus (Blattidae, Lamproblattinae). Tandem mass spectrometry revealed the phosphorylation site as Ser(5). Phosphorylated CAPA pyrokinin was then also detected in most other cockroach lineages (e. g. Blaberidae, Polyphagidae) but not in closely related blattid species such as Periplaneta americana. This is remarkable since the sequence of CAPA pyrokinin is identical in Lamproblatta and Periplaneta. A consensus sequence of CAPA pyrokinins of cockroaches revealed a conserved motif that suggests phosphorylation by a Four-jointed/FAM20C related kinase. (C) 2014 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sturm, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-434644
DOI:	10.1016/j.peptides.2014.04.012
Journal or Publication Title:	Peptides
Volume:	57
Page Range:	S. 52 - 59
Date:	2014
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1873-5169
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADULT DROSOPHILA-MELANOGASTER; MEDULLARY CHROMAFFIN GRANULES; CENTRAL-NERVOUS-SYSTEM; PERISYMPATHETIC ORGANS; NEUROPEPTIDE; PEPTIDES; SEQUENCE; KINASE; IDENTIFICATION; PEPTIDOMICS Multiple languages Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43464