Braeutigam, Andrea, Schliesky, Simon, Kuelahoglu, Canan, Osborne, Colin P. and Weber, Andreas P. M. (2014). Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. J. Exp. Bot., 65 (13). S. 3579 - 3594. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431
Full text not available from this repository.Abstract
C-4 photosynthesis affords higher photosynthetic carbon conversion efficiency than C-3 photosynthesis and it therefore represents an attractive target for engineering efforts aiming to improve crop productivity. To this end, blueprints are required that reflect C-4 metabolism as closely as possible. Such blueprints have been derived from comparative transcriptome analyses of C-3 species with related C-4 species belonging to the NAD-malic enzyme (NAD-ME) and NADP-ME subgroups of C-4 photosynthesis. However, a comparison between C-3 and the phosphoenolpyruvate carboxykinase (PEP-CK) subtype of C-4 photosynthesis is still missing. An integrative analysis of all three C-4 subtypes has also not been possible to date, since no comparison has been available for closely related C-3 and PEP-CK C-4 species. To generate the data, the guinea grass Megathyrsus maximus, which represents a PEP-CK species, was analysed in comparison with a closely related C-3 sister species, Dichanthelium clandestinum, and with publicly available sets of RNA-Seq data from C-4 species belonging to the NAD-ME and NADP-ME subgroups. The data indicate that the core C-4 cycle of the PEP-CK grass M. maximus is quite similar to that of NAD-ME species with only a few exceptions, such as the subcellular location of transfer acid production and the degree and pattern of up-regulation of genes encoding C-4 enzymes. One additional mitochondrial transporter protein was associated with the core cycle. The broad comparison identified sucrose and starch synthesis, as well as the prevention of leakage of C-4 cycle intermediates to other metabolic pathways, as critical components of C-4 metabolism. Estimation of intercellular transport fluxes indicated that flux between cells is increased by at least two orders of magnitude in C-4 species compared with C-3 species. In contrast to NAD-ME and NADP-ME species, the transcription of photosynthetic electron transfer proteins was unchanged in PEP-CK. In summary, the PEP-CK blueprint of M. maximus appears to be simpler than those of NAD-ME and NADP-ME plants.
Item Type: | Journal Article | ||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-435323 | ||||||||||||||||||||||||
DOI: | 10.1093/jxb/eru100 | ||||||||||||||||||||||||
Journal or Publication Title: | J. Exp. Bot. | ||||||||||||||||||||||||
Volume: | 65 | ||||||||||||||||||||||||
Number: | 13 | ||||||||||||||||||||||||
Page Range: | S. 3579 - 3594 | ||||||||||||||||||||||||
Date: | 2014 | ||||||||||||||||||||||||
Publisher: | OXFORD UNIV PRESS | ||||||||||||||||||||||||
Place of Publication: | OXFORD | ||||||||||||||||||||||||
ISSN: | 1460-2431 | ||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/43532 |
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