Universität zu Köln

Livnat-Levanon, Nurit, Kevei, Eva ORCID: 0000-0002-0560-9208, Kleifeld, Oded, Krutauz, Daria, Segref, Alexandra, Rinaldi, Teresa ORCID: 0000-0001-6291-245X, Erpapazoglou, Zoi, Cohen, Mickael ORCID: 0000-0002-1372-680X, Reis, Noa, Hoppe, Thorsten ORCID: 0000-0002-4734-9352 and Glickman, Michael H. (2014). Reversible 26S Proteasome Disassembly upon Mitochondrial Stress. Cell Reports, 7 (5). S. 1371 - 1381. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

In eukaryotic cells, proteasomes exist primarily as 26S holoenzymes, the most efficient configuration for ubiquitinated protein degradation. Here, we show that acute oxidative stress caused by environmental insults or mitochondrial defects results in rapid disassembly of 26S proteasomes into intact 20S core and 19S regulatory particles. Consequently, polyubiquitinated substrates accumulate, mitochondrial networks fragment, and cellular reactive oxygen species (ROS) levels increase. Oxidation of cysteine residues is sufficient to induce proteasome disassembly, and spontaneous reassembly from existing components is observed both in vivo and in vitro upon reduction. Ubiquitin-dependent substrate turnover also resumes after treatment with antioxidants. Reversible attenuation of 26S proteasome activity induced by acute mitochondrial or oxidative stress may be a short-term response distinct from adaptation to long-term ROS exposure or changes during aging.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Livnat-Levanon, Nurit UNSPECIFIED UNSPECIFIED UNSPECIFIED Kevei, Eva UNSPECIFIED orcid.org/0000-0002-0560-9208 UNSPECIFIED Kleifeld, Oded UNSPECIFIED UNSPECIFIED UNSPECIFIED Krutauz, Daria UNSPECIFIED UNSPECIFIED UNSPECIFIED Segref, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Rinaldi, Teresa UNSPECIFIED orcid.org/0000-0001-6291-245X UNSPECIFIED Erpapazoglou, Zoi UNSPECIFIED UNSPECIFIED UNSPECIFIED Cohen, Mickael UNSPECIFIED orcid.org/0000-0002-1372-680X UNSPECIFIED Reis, Noa UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoppe, Thorsten UNSPECIFIED orcid.org/0000-0002-4734-9352 UNSPECIFIED Glickman, Michael H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-435826
DOI:	10.1016/j.celrep.2014.04.030
Journal or Publication Title:	Cell Reports
Volume:	7
Number:	5
Page Range:	S. 1371 - 1381
Date:	2014
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 20S PROTEASOME; SACCHAROMYCES-CEREVISIAE; OXIDATIVE STRESS; NEURODEGENERATIVE DISEASES; REGULATORY PARTICLE; OXIDIZED PROTEINS; DEPENDENT DEGRADATION; QUALITY-CONTROL; UBIQUITIN; SYSTEM Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43582