Universität zu Köln

Swaminathan, Karthic ORCID: 0000-0002-0981-7549, Mueller-Taubenberger, Annette, Faix, Jan, Rivero, Francisco ORCID: 0000-0001-5435-6991 and Noegel, Angelika A. (2014). A Cdc42-and Rac-interactive binding (CRIB) domain mediates functions of coronin. Proc. Natl. Acad. Sci. U. S. A., 111 (1). S. E25 - 9. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

The Cdc42- and Rac-interactive binding motif (CRIB) of coronin binds to Rho GTPases with a preference for GDP-loaded Rac. Mutation of the Cdc42- and Rac-interactive binding motif abrogates Rac binding. This results in increased 1evels of activated Rac in coronin-deficient Dictyostelium cells (corA(-)), which impacts myosin II assembly. corA(-) cells show increased accumulation of myosin II in the cortex of growth-phase cells. Myosin II assembly is regulated by myosin heavy chain kinase-mediated phosphorylation of its tail. Kinase activity depends on the activation state of the p21-activated kinase a. The myosin II defect of corA(-) mutant is alleviated by dominant-negative p21-activated kinase a. It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and Rac-interactive binding motif failed to rescue the myosin defect in corA(-) mutant cells. Ectopically expressed myosin heavy chain kinases affinity purified from corA(-) cells show reduced kinase activity. We propose that coronin through its affinity for GDP-Rac regulates the availability of GTP-Rac for activation of downstream effectors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Swaminathan, Karthic UNSPECIFIED orcid.org/0000-0002-0981-7549 UNSPECIFIED Mueller-Taubenberger, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Faix, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Rivero, Francisco UNSPECIFIED orcid.org/0000-0001-5435-6991 UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-448699
DOI:	10.1073/pnas.1315368111
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	111
Number:	1
Page Range:	S. E25 - 9
Date:	2014
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MYOSIN-II; DIFFERENTIAL LOCALIZATION; SIGNAL-TRANSDUCTION; ACTIVATING PROTEINS; ARP2/3 COMPLEX; RHO-GTPASES; ACTIN; CYTOKINESIS; DYNAMICS; FAMILY Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44869