Universität zu Köln

Schacherl, Magdalena ORCID: 0000-0002-5478-2509, Waltersperger, Sandro and Baumann, Ulrich (2013). Structural characterization of the ribonuclease H-like type ASKHA superfamily kinase MK0840 from Methanopyrus kandleri. Acta Crystallogr. Sect. D-Struct. Biol., 69. S. 2440 - 2451. CHESTER: INT UNION CRYSTALLOGRAPHY. ISSN 2059-7983

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Abstract

Murein recycling is a process in which microorganisms recover peptidoglycan-degradation products in order to utilize them in cell wall biosynthesis or basic metabolic pathways. Methanogens such as Methanopyrus kandleri contain pseudomurein, which differs from bacterial murein in its composition and branching. Here, four crystal structures of the putative sugar kinase MK0840 from M. kandleri in apo and nucleotide-bound states are reported. MK0840 shows high similarity to bacterial anhydro-N-acetylmuramic acid kinase, which is involved in murein recycling. The structure shares a common fold with panthothenate kinase and the 2-hydroxyglutaryl-CoA dehydratase component A, both of which are members of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of phosphotransferases. Local conformational changes in the nucleotide-binding site between the apo and holo forms are observed upon nucleotide binding. Further insight is given into domain movements and putative active-site residues are identified.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schacherl, Magdalena UNSPECIFIED orcid.org/0000-0002-5478-2509 UNSPECIFIED Waltersperger, Sandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-470675
DOI:	10.1107/S0907444913022683
Journal or Publication Title:	Acta Crystallogr. Sect. D-Struct. Biol.
Volume:	69
Page Range:	S. 2440 - 2451
Date:	2013
Publisher:	INT UNION CRYSTALLOGRAPHY
Place of Publication:	CHESTER
ISSN:	2059-7983
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MAIN MOLECULAR HINGE; L-RHAMNULOSE KINASE; CRYSTAL-STRUCTURES; PROTEIN-STRUCTURE; YEAST HEXOKINASE; GLYCEROL KINASE; ACETATE KINASE; ACTIVE-SITE; MECHANISM; MODEL Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47067