Universität zu Köln

Spallek, Thomas ORCID: 0000-0003-4425-1015, Beck, Martina, Ben Khaled, Sara, Salomon, Susanne, Bourdais, Gildas ORCID: 0000-0001-9409-1107, Schellmann, Swen and Robatzek, Silke (2013). ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity. PLoS Genet., 9 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Spallek, Thomas UNSPECIFIED orcid.org/0000-0003-4425-1015 UNSPECIFIED Beck, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Ben Khaled, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Salomon, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Bourdais, Gildas UNSPECIFIED orcid.org/0000-0001-9409-1107 UNSPECIFIED Schellmann, Swen UNSPECIFIED UNSPECIFIED UNSPECIFIED Robatzek, Silke UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-471629
DOI:	10.1371/journal.pgen.1004035
Journal or Publication Title:	PLoS Genet.
Volume:	9
Number:	12
Date:	2013
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7404
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GOLGI NETWORK/EARLY ENDOSOME; RECOGNITION RECEPTOR FLS2; PLASMA-MEMBRANE; INNATE IMMUNITY; ABSCISIC-ACID; MULTIVESICULAR BODIES; FLAGELLIN SENSING2; ARABIDOPSIS CELLS; ENDOCYTOSIS; TRAFFICKING Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47162