Universität zu Köln

Zeng, J., Kim, D., Li, K. -W., Sharp, K., Steward, O., Zaucke, F. and Luo, Z. D. (2013). Thrombospondin-4 contributes to spinal cord injury-induced changes in nociception. Eur. J. Pain, 17 (10). S. 1458 - 1465. HOBOKEN: WILEY. ISSN 1532-2149

Full text not available from this repository.

Abstract

BackgroundOur previous data have indicated that nerve injury-induced up-regulation of thrombospondin-4 (TSP4) proteins in dorsal spinal cord plays a causal role in neuropathic pain state development in a spinal nerve ligation model. To investigate whether TSP4 proteins also contribute to the development of centrally mediated changes in nociception after spinal cord injury (SCI), we investigated whether SCI induced TSP4 dysregulation, and if so, whether this change correlated with changes in nociception in a T-9 spinal cord contusion injury model. MethodsBehavioural sensitivity to mechanical, thermal stimuli and locomotor function recovery were tested blindly in SCI or sham rats post-injury. Intrathecal antisense or mismatch control oligodeoxynucleotides were used to treat SCI rats with nociceptive hyperreflexia, and Western blots were used to measure TSP4 protein levels in dorsal spinal cord samples. ResultsSCI induced below-level hindpaw hypersensitivity to stimuli. TSP4 protein levels are up-regulated in dorsal spinal cord of SCI rats with nociceptive hyperreflexia, but not in SCI rats without nociceptive hyperreflexia. There was no significant difference in motor function recovery post-injury between SCI rats with or without nociceptive hyperreflexia. Intrathecal treatment with TSP4 antisense, but not mismatch control, oligodeoxynucleotides led to reversal of injury-induced TSP4 up-regulation and nociceptive hyperreflexia in SCI rats. ConclusionsSCI leads to TSP4 up-regulation in lumbar spinal cord that may play a critical role in mediating centrally mediated behavioural hypersensitivity. Blocking this pathway may be helpful in management of SCI-induced changes in nociception.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zeng, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kim, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, K. -W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sharp, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Steward, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Z. D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-473161
DOI:	10.1002/j.1532-2149.2013.00326.x
Journal or Publication Title:	Eur. J. Pain
Volume:	17
Number:	10
Page Range:	S. 1458 - 1465
Date:	2013
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1532-2149
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUBUNIT UP-REGULATION; NEUROPATHIC PAIN; DORSAL-HORN; ALLODYNIA; MODEL Multiple languages Anesthesiology; Clinical Neurology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47316