Universität zu Köln

Spelten, Oliver, Wetsch, Wolfgang A., Wrettos, Georg, Kalenka, Armin and Hinkelbein, Jochen (2013). Response of rat lung tissue to short-term hyperoxia: a proteomic approach. Mol. Cell. Biochem., 383 (1-2). S. 231 - 243. DORDRECHT: SPRINGER. ISSN 1573-4919

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Abstract

An inspiratory oxygen fraction of 1.0 is often required to avoid hypoxia both in many pre- and in-hospital situations. On the other hand, hyperoxia may lead to deleterious consequences (cell growth inhibition, inflammation, and apoptosis) for numerous tissues including the lung. Whereas clinical effects of hyperoxic lung injury are well known, its impact on the expression of lung proteins has not yet been evaluated sufficiently. The aim of this study was to analyze time-dependent alterations of protein expression in rat lung tissue after short-term normobaric hyperoxia (NH). After approval of the local ethics committee for animal research, N = 36 Wistar rats were randomized into six different groups: three groups with NH with exposure to 100 % oxygen for 3 h and three groups with normobaric normoxia (NN) with exposure to room air (21 % oxygen). After the end of the experiments, lungs were removed immediately (NH0 and NN0), after 3 days (NH3 and NN3) and after 7 days (NH7 and NN7). Lung lysates were analyzed by two-dimensional gel electrophoresis (2D-GE) followed by peptide mass fingerprinting using mass spectrometry. Statistical analysis was performed with Delta 2D (DECODON GmbH, Greifswald, Germany; ANOVA, Bonferroni correction, p < 0.01). Biological functions of differential regulated proteins were studied using functional network analysis (Ingenuity Pathways Analysis, IPA). pO(2) was significantly higher in NH-groups compared to NN-groups (581 +/- A 28 vs. 98 +/- A 12 mmHg; p < 0.01), all other physiological parameters did not differ. Expression of 14 proteins were significantly altered: two proteins were up-regulated and 12 proteins were down-regulated. Even though NH was comparatively short termed, significant alterations in lung protein expression could be demonstrated up to 7 days after hyperoxia. The identified proteins indicate an association with cell growth inhibition, regulation of apoptosis, and approval of structural cell integrity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Spelten, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Wetsch, Wolfgang A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wrettos, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Kalenka, Armin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hinkelbein, Jochen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-473183
DOI:	10.1007/s11010-013-1771-y
Journal or Publication Title:	Mol. Cell. Biochem.
Volume:	383
Number:	1-2
Page Range:	S. 231 - 243
Date:	2013
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-4919
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEAT-SHOCK PROTEINS; INDUCED CELL-DEATH; REACTIVE OXYGEN; SUPEROXIDE-DISMUTASE; RESPIRATORY-DISTRESS; NERVOUS-SYSTEM; EXPRESSION; HSP27; PROTECTS; STRESS Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47318