Universität zu Köln

Persigehl, Thorsten, Ring, Janine, Budny, Tymoteusz, Hahnenkamp, Anke, Stoeppeler, Sandra, Schwartz, Lawrence H., Spiegel, Hans-Ullrich, Heindel, Walter, Remmele, Stefanie and Bremer, Christoph (2013). Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy. Mol. Imaging, 12 (7). THOUSAND OAKS: SAGE PUBLICATIONS INC. ISSN 1536-0121

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Abstract

The aim of this study was to evaluate a robust magnetic resonance (MR) vessel size imaging (VSI) method for the noninvasive assessment of mean vessel size in solid tumors in a clinical dose range of ultrasmall superparamagnetic particles of iron oxide (USPIO). Therefore, USPIO-enhanced MR-VSI was performed on DU-4475, MDA-MB-435, and EOMA tumor-bearing mice xenografts with known differences in angiogenic activity and vessel morphology. MR results were compared to vessel sizes determined by immunohistochemistry (anti-CD31) and by intravital microscopy (IVM). MR-VSI revealed significantly different mean vessel sizes between the xenograft models at both USPIO doses (DU-4475: 20.6 +/- 4.9 mu m; MDA-MB-435: 37.4 +/- 8.8 mu m; and EOMA: 60.3 +/-9.6 mu m at 80 mu mol/kg; p < .05). Immunohistochemistry revealed lower values for all tumor entities, whereas the size distribution was in line with MR-measurements. IVM corroborated the MR results for DU-4475 and MDA-MB435, but showed similar vessel sizes for MDA-MB-435 and EOMA. Our MR-VSI method allowed a noninvasive estimation of the mean vessel size in mice xenograft solid tumors with variable vascularity using a clinically relevant USPIO dose range.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Persigehl, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Ring, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Budny, Tymoteusz UNSPECIFIED UNSPECIFIED UNSPECIFIED Hahnenkamp, Anke UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoeppeler, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwartz, Lawrence H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Spiegel, Hans-Ullrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindel, Walter UNSPECIFIED UNSPECIFIED UNSPECIFIED Remmele, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Bremer, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-474308
DOI:	10.2310/7290.2013.00059
Journal or Publication Title:	Mol. Imaging
Volume:	12
Number:	7
Date:	2013
Publisher:	SAGE PUBLICATIONS INC
Place of Publication:	THOUSAND OAKS
ISSN:	1536-0121
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VIVO; BLOOD-VOLUME; ANGIOGENESIS; CANCER; MODEL; QUANTIFICATION; ANGIOGRAPHY; METASTASES; PARTICLES; 1ST-PASS Multiple languages Biochemical Research Methods; Radiology, Nuclear Medicine & Medical Imaging Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47430