Universität zu Köln

Schmitt, Markus ORCID: 0000-0002-7827-6694, Wieland, Ulrike, Kreuter, Alexander and Pawlita, Michael ORCID: 0000-0002-4720-8306 (2012). C-terminal deletions of Merkel cell polyomavirus large T-antigen, a highly specific surrogate marker for virally induced malignancy. Int. J. Cancer, 131 (12). S. 2863 - 2869. HOBOKEN: WILEY-BLACKWELL. ISSN 0020-7136

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Abstract

In 67100% of cutaneous Merkel cell carcinomas (MCC) the Merkel cell polyomavirus (MCPyV) integrates into the host genome. Mutations and deletions truncating the C-terminal helicase domain of the T-antigen (TAg) protein have been detected in these MCCs, but not in healthy skin specimens. C-terminal deletions of the TAg nucleic acid sequences are characteristic for about 38% of these cases. While the association of MCPyV with MCC has been proven, it is unknown whether MCPyV may play a similar role in other tumor entities. We describe in detail the development and validation of a novel Merkel cell polyomavirus TAg C-terminus deletion assay (MCPyV Delta C-TAg). The triplex real-time PCR quantifies the N- and C-terminal part of the MCPyV TAg gene and the cellular beta-globin gene. By comparing the copy numbers of the N- and C-terminal part, deletions of the MCPyV TAg C-terminus are rapidly identified. MCPyV Delta C-TAg was used to assess the physical state of MCPyV TAg in a large series of 55 MCCs, 15 cutaneous lymphomas and 47 forehead smears of healthy individuals. Neither DNA positivity nor viral load was able to discriminate MCCs from the other different types of samples. However, deleted TAg C-terminus sequences were detected only in MCPyV positive MCCs (39%). Consequently, detection of deleted C-terminal TAg sequences appears to be a highly specific surrogate marker for virally induced malignancy and should be used to support novel assumed MCPyVtumor associations. The study further supports the notion that MCPyV does not play a role in cutaneous lymphoma pathogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schmitt, Markus UNSPECIFIED orcid.org/0000-0002-7827-6694 UNSPECIFIED Wieland, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreuter, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Pawlita, Michael UNSPECIFIED orcid.org/0000-0002-4720-8306 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476673
DOI:	10.1002/ijc.27607
Journal or Publication Title:	Int. J. Cancer
Volume:	131
Number:	12
Page Range:	S. 2863 - 2869
Date:	2012
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	0020-7136
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARCINOMA; INFECTION; SKIN; DNA; MCV; INTEGRATION; LYMPHOMA; TISSUES; CANCER; TUMORS Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47667