Universität zu Köln

Gruteser, Nadine, Marin, Kay, Kraemer, Reinhard and Thomas, Gavin H. (2012). Sialic acid utilization by the soil bacterium Corynebacterium glutamicum. FEMS Microbiol. Lett., 336 (2). S. 131 - 139. HOBOKEN: WILEY-BLACKWELL. ISSN 0378-1097

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Abstract

The ability to use the sialic acid, N-acetylneuraminic acid, Neu5Ac, as a nutrient has been characterized in a number of bacteria, most of which are human pathogens that encounter this molecule because of its presence on mucosal surfaces. The soil bacterium Corynebacterium glutamicum also has a full complement of genes for sialic acid catabolism, and we demonstrate that it can use Neu5Ac as a sole source of carbon and energy and isolate mutants with a much reduced growth lag on Neu5Ac. Disruption of the cg2937 gene, encoding a component of a predicted sialic acid-specific ABC transporter, results in a complete loss of growth of C. glutamicum on Neu5Ac and also a complete loss of [14C]-Neu5Ac uptake into cells. Uptake of [14C]-Neu5Ac is induced by pregrowth on Neu5Ac, but the additional presence of glucose prevents this induction. The demonstration that a member of the Actinobacteria can transport and catabolize Neu5Ac efficiently suggests that sialic acid metabolism has a physiological role in the soil environment.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gruteser, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraemer, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Gavin H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-480023
DOI:	10.1111/j.1574-6968.2012.02663.x
Journal or Publication Title:	FEMS Microbiol. Lett.
Volume:	336
Number:	2
Page Range:	S. 131 - 139
Date:	2012
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	0378-1097
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COMPLETE GENOME SEQUENCE; HAEMOPHILUS-INFLUENZAE; ESCHERICHIA-COLI; TRAP TRANSPORTER; VIBRIO-CHOLERAE; BINDING-SITES; AMINO-ACIDS; IDENTIFICATION; DIPHTHERIAE; GENE Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48002