Universität zu Köln

Peleg, Anton Y., de Breij, Anna, Adams, Mark D., Cerqueira, Gustavo M., Mocali, Stefano ORCID: 0000-0002-1173-7206, Galardini, Marco ORCID: 0000-0003-2018-8242, Nibbering, Peter H., Earl, Ashlee M., Ward, Doyle V., Paterson, David L., Seifert, Harald and Dijkshoorn, Lenie (2012). The Success of Acinetobacter Species; Genetic, Metabolic and Virulence Attributes. PLoS One, 7 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

An understanding of why certain Acinetobacter species are more successful in causing nosocomial infections, transmission and epidemic spread in healthcare institutions compared with other species is lacking. We used genomic, phenotypic and virulence studies to identify differences between Acinetobacter species. Fourteen strains representing nine species were examined. Genomic analysis of six strains showed that the A. baumannii core genome contains many genes important for diverse metabolism and survival in the host. Most of the A. baumannii core genes were also present in one or more of the less clinically successful species. In contrast, when the accessory genome of an individual A. baumannii strain was compared to a strain of a less successful species (A. calcoaceticus RUH2202), many operons with putative virulence function were found to be present only in the A. baumannii strain, including the csu operon, the acinetobactin chromosomal cluster, and bacterial defence mechanisms. Phenotype microarray analysis showed that compared to A. calcoaceticus (RUH2202), A. baumannii ATCC 19606(T) was able to utilise nitrogen sources more effectively and was more tolerant to pH, osmotic and antimicrobial stress. Virulence differences were also observed, with A. baumannii ATCC 19606(T), A. pittii SH024, and A. nosocomialis RUH2624 persisting and forming larger biofilms on human skin than A. calcoaceticus. A. baumannii ATCC 19606 T and A. pittii SH024 were also able to survive in a murine thigh infection model, whereas the other two species were eradicated. The current study provides important insights into the elucidation of differences in clinical relevance among Acinetobacter species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Peleg, Anton Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED de Breij, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Adams, Mark D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cerqueira, Gustavo M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mocali, Stefano UNSPECIFIED orcid.org/0000-0002-1173-7206 UNSPECIFIED Galardini, Marco UNSPECIFIED orcid.org/0000-0003-2018-8242 UNSPECIFIED Nibbering, Peter H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Earl, Ashlee M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ward, Doyle V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Paterson, David L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Dijkshoorn, Lenie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-480846
DOI:	10.1371/journal.pone.0046984
Journal or Publication Title:	PLoS One
Volume:	7
Number:	10
Date:	2012
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SKIN EQUIVALENT; MOLECULAR CHARACTERIZATION; BAUMANNII COMPLEX; BIOFILM FORMATION; GENOME SEQUENCE; IDENTIFICATION; SIDEROPHORE; ACQUISITION; EVOLUTION; ADHERENCE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48084