Universität zu Köln

Koenig, U. and Borcherding, J. (2012). Preserving young-of-the-year Perca fluviatilis in ethanol, formalin, or in a frozen state and the consequences for measuring morphometrics. J. Appl. Ichthyol., 28 (5). S. 740 - 745. HOBOKEN: WILEY. ISSN 1439-0426

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Abstract

As it is often not possible to immediately analyse individuals sampled in the field, captured fish are preserved and stored for later investigation. The objective of this study was to assess the effects of various preservation methods on subsequent changes in morphometric parameters while also providing correction factors to re-calculate the original body dimensions when sampled fish are measured at a later date. In this study, juvenile perch (Perca fluviatilis, 66.5 +/- 10.2 mm total length) were measured directly after capture, then either frozen at -20 degrees C, preserved in 70% ethanol, or in 4% formalin. They were again measured after 24 h, 3 days, 7 days, and thereafter on a weekly basis for 8 weeks. Ethanol-preserved perch greatly decreased length and weight; formalin preservation also led to a comparable length reduction, but increased the weight of the perch. In contrast, frozen perch showed less shrinkage and only moderate weight loss. Of the three preservation methods, freezing clearly caused the fewest distortions. Hence, freezing is recommend as the most preferable preservation method, especially in multi-disciplinary studies on fish ecology; for all other preservation methods the correction factor over time will have to be pre-determined for each species and size class.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koenig, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Borcherding, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481712
DOI:	10.1111/j.1439-0426.2012.01958.x
Journal or Publication Title:	J. Appl. Ichthyol.
Volume:	28
Number:	5
Page Range:	S. 740 - 745
Date:	2012
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1439-0426
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LENGTH CHANGES; PRESERVATION TECHNIQUE; PHENOTYPIC PLASTICITY; MALLOTUS-VILLOSUS; WEIGHT; LARVAE; SHRINKAGE; BODY; MORPHOLOGY; GROWTH Multiple languages Fisheries; Marine & Freshwater Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48171