Universität zu Köln

Lu, Wenshu, Schneider, Maria, Neumann, Sascha, Jaeger, Verena-Maren, Taranum, Surayya, Munck, Martina, Cartwright, Sarah, Richardson, Christine ORCID: 0000-0003-2622-2261, Carthew, James, Noh, Kowoon ORCID: 0000-0003-3474-9215, Goldberg, Martin, Noegel, Angelika A. and Karakesisoglou, Iakowos (2012). Nesprin interchain associations control nuclear size. Cell. Mol. Life Sci., 69 (20). S. 3493 - 3510. BASEL: SPRINGER BASEL AG. ISSN 1420-9071

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Abstract

Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and associates instead to plectin, which binds intermediate filaments. Nesprins are integrated into the outer nuclear membrane via their C-terminal KASH-domain. Here, we show that nesprin-1/-2 ABDs physically and functionally interact with nesprin-3. Thus, both ends of nesprin-1/-2 giant are integrated at the nuclear surface: via the C-terminal KASH-domain and the N-terminal ABD-nesprin-3 association. Interestingly, nesprin-2 ABD or KASH-domain overexpression leads to increased nuclear areas. Conversely, nesprin-2 mini (contains the ABD and KASH-domain but lacks the massive nesprin-2 giant rod segment) expression yields smaller nuclei. Nuclear shrinkage is further enhanced upon nesprin-3 co-expression or microfilament depolymerization. Our findings suggest that multivariate intermolecular nesprin interactions with the cytoskeleton form a lattice-like filamentous network covering the outer nuclear membrane, which determines nuclear size.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lu, Wenshu UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaeger, Verena-Maren UNSPECIFIED UNSPECIFIED UNSPECIFIED Taranum, Surayya UNSPECIFIED UNSPECIFIED UNSPECIFIED Munck, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Cartwright, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Richardson, Christine UNSPECIFIED orcid.org/0000-0003-2622-2261 UNSPECIFIED Carthew, James UNSPECIFIED UNSPECIFIED UNSPECIFIED Noh, Kowoon UNSPECIFIED orcid.org/0000-0003-3474-9215 UNSPECIFIED Goldberg, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karakesisoglou, Iakowos UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-482266
DOI:	10.1007/s00018-012-1034-1
Journal or Publication Title:	Cell. Mol. Life Sci.
Volume:	69
Number:	20
Page Range:	S. 3493 - 3510
Date:	2012
Publisher:	SPRINGER BASEL AG
Place of Publication:	BASEL
ISSN:	1420-9071
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DREIFUSS MUSCULAR-DYSTROPHY; MEMBRANE PROTEIN; ENVELOPE ARCHITECTURE; DOMAIN PROTEINS; ACTIN FLOW; MIGRATION; SPECTRIN; LOCALIZATION; INTERACTS; LAMINS Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48226