Universität zu Köln

Rybniker, Jan, Krumbach, Karin, van Gumpel, Edeltraud, Plum, Georg, Eggeling, Lothar and Hartmann, Pia (2011). The cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532, an L-serine dehydratase of Mycobacterium smegmatis. J. Basic Microbiol., 51 (5). S. 515 - 523. HOBOKEN: WILEY. ISSN 1521-4028

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Abstract

Mycobacteriophage L5 is a temperate phage infecting a broad range of mycobacterial species. Upon induction of lytic growth, L5 rapidly switches off host protein synthesis. We have recently identified the mycobacteriophage L5 early protein gp77 as a host shut-off protein that acts growth inhibitory in the mycobacterial host when expressed through the corresponding phage promoter. Here we present data showing that this purified phage protein of unknown function specifically binds to protein MSMEG_3532 when incubated with cell lysates of Mycobacterium smegmatis. This interaction was confirmed by pull-down assays using purified MSMEG_3532 as bait which co-purified with gp77. The amino acid sequence of MSMEG_3532 is nearly identical to that of threonine dehydratases, serine dehydratases and an L-threo-3-hydroxyaspartate dehydratase. An enzymatic assay identified this host protein as a pyridoxal-5'-phosphate-dependent L-serine dehydratase (SdhA) which converts L-serine to pyruvate. This is the first biochemical characterization of a SdhA derived from mycobacteria. Though the addition of purified gp77 to the established in vitro assay had no influence on SdhA activity at a saturating L-serine concentration, the specific interaction of phage protein and dehydratase in vivo may well have a role in altering the amino acid pool or the products of amino acid metabolism in favour of phage maturation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rybniker, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Krumbach, Karin UNSPECIFIED UNSPECIFIED UNSPECIFIED van Gumpel, Edeltraud UNSPECIFIED UNSPECIFIED UNSPECIFIED Plum, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Eggeling, Lothar UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-488497
DOI:	10.1002/jobm.201000446
Journal or Publication Title:	J. Basic Microbiol.
Volume:	51
Number:	5
Page Range:	S. 515 - 523
Date:	2011
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1521-4028
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IDENTIFICATION; GROWTH; EXPRESSION; CLONING; ACID Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48849