Universität zu Köln

Klatt, Andreas R., Klinger, Gabriele, Paul-Klausch, Brigitte, Renno, Joerg H., Schmidt, Joachim, Malchau, Gebhart and Wielckens, Klaus (2010). TAK1 mediates the collagen-II-dependent induction of the COX-2 gene and PGE2 release in primary human chondrocytes. Connect. Tissue Res., 51 (6). S. 452 - 459. LONDON: INFORMA HEALTHCARE. ISSN 0300-8207

Full text not available from this repository.

Abstract

We investigated the role of transforming growth factor-beta activated kinase 1 (TAK1) in collagen II signaling in primary human chondrocytes (PHCs). We asked whether TAK1 acts as a modulator of collagen II signaling with respect to collagen-II-dependent induction of cyclooxigenase-2 (COX-2) in PHCs and release of PGE2 from PHCs. Therefore, PHCs were incubated with collagen II, and cells were then analyzed by RT-PCR for the expression of COX-2. ELISA was used to quantify PGE2 release. To examine the influence of TAK1 on these events, TAK1 gene silencing was performed by RNAi in PHCs prior to collagen II treatment. Results indicated that COX-2 gene expression and PGE2 release are specific outcomes of collagen II signaling and that both depend on TAK1 mediation. These findings are promising in that therapeutic inhibition of TAK1 might be used to reduce pain and relieve inflammatory symptoms that are common in osteoarthritis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klatt, Andreas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Klinger, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Paul-Klausch, Brigitte UNSPECIFIED UNSPECIFIED UNSPECIFIED Renno, Joerg H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Malchau, Gebhart UNSPECIFIED UNSPECIFIED UNSPECIFIED Wielckens, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491299
DOI:	10.3109/03008201003668360
Journal or Publication Title:	Connect. Tissue Res.
Volume:	51
Number:	6
Page Range:	S. 452 - 459
Date:	2010
Publisher:	INFORMA HEALTHCARE
Place of Publication:	LONDON
ISSN:	0300-8207
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYNOVIAL TISSUES; JOINT DISEASES; IN-VITRO; CYCLOOXYGENASE-2; KINASE; EXPRESSION; CARTILAGE; ARTHRITIS; PROSTAGLANDINS; INTERLEUKIN-6 Multiple languages Cell Biology; Orthopedics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49129