Universität zu Köln

Kalkhof, Stefan ORCID: 0000-0001-6121-7105, Haehn, Sebastian, Paulsson, Mats, Smyth, Neil ORCID: 0000-0002-3734-2149, Meiler, Jens ORCID: 0000-0001-8945-193X and Sinz, Andrea (2010). Computational modeling of laminin N-terminal domains using sparse distance constraints from disulfide bonds and chemical cross-linking. Proteins, 78 (16). S. 3409 - 3428. HOBOKEN: WILEY. ISSN 1097-0134

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Abstract

Basement membranes are thin extracellular protein layers, which separate endothelial and epithelial cells from the underlying connecting tissue. The main noncollagenous components of basement membranes are laminins, trimeric glycoproteins, which form polymeric networks by interactions of their N-terminal (LN) domains; however, no high-resolution structure of laminin LN domains exists so far. To construct models for laminin beta(1) and gamma(1) LN domains, 14 potentially suited template structures were determined using fold recognition methods. For each target/template-combination comparative models were created with Rosetta. Final models were selected based on their agreement with experimentally obtained distance constraints from natural cross-links, that is, disulfide bonds as well as chemical cross-links obtained from reactions with two amine-reactive cross-linkers. We predict that laminin beta(1) and gamma(1) LN domains share the galactose-binding domain-like fold.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalkhof, Stefan UNSPECIFIED orcid.org/0000-0001-6121-7105 UNSPECIFIED Haehn, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Smyth, Neil UNSPECIFIED orcid.org/0000-0002-3734-2149 UNSPECIFIED Meiler, Jens UNSPECIFIED orcid.org/0000-0001-8945-193X UNSPECIFIED Sinz, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-492022
DOI:	10.1002/prot.22848
Journal or Publication Title:	Proteins
Volume:	78
Number:	16
Page Range:	S. 3409 - 3428
Date:	2010
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1097-0134
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-STRUCTURE DETERMINATION; RESONANCE MASS-SPECTROMETRY; CRYSTAL-STRUCTURE; STRUCTURE PREDICTION; EXTRACELLULAR DOMAIN; FOLD RECOGNITION; SCORING FUNCTION; STRUCTURAL BASIS; C2 DOMAIN; BINDING Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49202