Universität zu Köln

Depmeier, Hannah ORCID: 0000-0001-9825-3134, Hoffmann, Eva, Bornewasser, Lisa and Kath-Schorr, Stephanie ORCID: 0000-0002-5180-360X (2021). Strategies for Covalent Labeling of Long RNAs. ChemBioChem, 22 (19). S. 2826 - 2848. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1439-7633

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Abstract

The introduction of chemical modifications into long RNA molecules at specific positions for visualization, biophysical investigations, diagnostic and therapeutic applications still remains challenging. In this review, we present recent approaches for covalent internal labeling of long RNAs. Topics included are the assembly of large modified RNAs via enzymatic ligation of short synthetic oligonucleotides and synthetic biology approaches preparing site-specifically modified RNAs via in vitro transcription using an expanded genetic alphabet. Moreover, recent approaches to employ deoxyribozymes (DNAzymes) and ribozymes for RNA labeling and RNA methyltransferase based labeling strategies are presented. We discuss the potentials and limits of the individual methods, their applicability for RNAs with several hundred to thousands of nucleotides in length and indicate future directions in the field.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Depmeier, Hannah UNSPECIFIED orcid.org/0000-0001-9825-3134 UNSPECIFIED Hoffmann, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Bornewasser, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Kath-Schorr, Stephanie UNSPECIFIED orcid.org/0000-0002-5180-360X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-561599
DOI:	10.1002/cbic.202100161
Journal or Publication Title:	ChemBioChem
Volume:	22
Number:	19
Page Range:	S. 2826 - 2848
Date:	2021
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1439-7633
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language UNNATURAL BASE-PAIR; SITE-SPECIFIC INCORPORATION; ALPHABET EXPANSION TRANSCRIPTION; AZIDE-ALKYNE CYCLOADDITION; DIELS-ALDER REACTIONS; MESSENGER-RNA; ENZYMATIC INCORPORATION; GENETIC ALPHABET; CLICK CHEMISTRY; IN-VITRO Multiple languages Biochemistry & Molecular Biology; Chemistry, Medicinal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56159