Universität zu Köln

Glowa, Dorothea, Comelli, Petra, Chandler, John W. and Werr, Wolfgang (2021). Clonal sector analysis and cell ablation confirm a function for DORNROESCHEN-LIKE in founder cells and the vasculature in Arabidopsis. Planta, 253 (2). NEW YORK: SPRINGER. ISSN 1432-2048

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Abstract

Main conclusion Inducible lineage analysis and cell ablation via conditional toxin expression in cells expressing the DORNRoSCHEN-LIKE transcription factor represent an effective and complementary adjunct to conventional methods of functional gene analysis. Classical methods of functional gene analysis via mutational and expression studies possess inherent limitations, and therefore, the function of a large proportion of transcription factors remains unknown. We have employed two complementary, indirect methods to obtain functional information for the AP2/ERF transcription factor DORNRoSCHEN-LIKE (DRNL), which is dynamically expressed in flowers and marks lateral organ founder cells. An inducible, two-component Cre-Lox system was used to express beta-glucuronidase GUS in cells expressing DRNL, to perform a sector analysis that reveals lineages of cells that transiently expressed DRNL throughout plant development. In a complementary approach, an inducible system was used to ablate cells expressing DRNL using diphtheria toxin A chain, to visualise the phenotypic consequences. These complementary analyses demonstrate that DRNL functionally marks founder cells of leaves and floral organs. Clonal sectors also included the vasculature of the leaves and petals, implicating a previously unidentified role for DRNL in provasculature development, which was confirmed in cotyledons by closer analysis of drnl mutants. Our findings demonstrate that inducible gene-specific lineage analysis and cell ablation via conditional toxin expression represent an effective and informative adjunct to conventional methods of functional gene analysis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Glowa, Dorothea UNSPECIFIED UNSPECIFIED UNSPECIFIED Comelli, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Chandler, John W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Werr, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-562689
DOI:	10.1007/s00425-020-03545-5
Journal or Publication Title:	Planta
Volume:	253
Number:	2
Date:	2021
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-2048
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SHOOT APICAL MERISTEM; GENETIC ABLATION; DORNROSCHEN-LIKE; AUXIN BIOSYNTHESIS; FLOWER DEVELOPMENT; LEAF DEVELOPMENT; FATE MAP; EXPRESSION; THALIANA; INITIATION Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56268