Universität zu Köln

Ros, Uris, Pedrera, Lohans and Garcia-Saez, Ana J. (2021). Techniques for studying membrane pores. Curr. Opin. Struct. Biol., 69. S. 108 - 117. LONDON: CURRENT BIOLOGY LTD. ISSN 1879-033X

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Abstract

Pore-forming proteins (PFPs) are of special interest because of the association of their activity with the disruption of the membrane impermeability barrier and cell death. They generally convert from a monomeric, soluble form into trans-membrane oligomers that induce the opening of membrane pores. The study of pore formation in membranes with molecular detail remains a challenging endeavor because of its highly dynamic and complex nature, usually involving diverse oligomeric structures with different functionalities. Here we discuss current methods applied for the structural and functional characterization of PFPs at the individual vesicle and cell level. We highlight how the development of high-resolution and single-molecule imaging techniques allows the analysis of the structural organization of protein oligomers and pore entities in lipid membranes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ros, Uris UNSPECIFIED UNSPECIFIED UNSPECIFIED Pedrera, Lohans UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Saez, Ana J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-565293
DOI:	10.1016/j.sbi.2021.03.012
Journal or Publication Title:	Curr. Opin. Struct. Biol.
Volume:	69
Page Range:	S. 108 - 117
Date:	2021
Publisher:	CURRENT BIOLOGY LTD
Place of Publication:	LONDON
ISSN:	1879-033X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EQUINATOXIN-II; TOXIN; BAX; FLUORESCENCE; MECHANISM; PROTEINS; REVEALS Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56529