Universität zu Köln

Gessner, Isabel ORCID: 0000-0001-9674-161X, Fries, Jochen W. U., Brune, Veronika ORCID: 0000-0001-5909-621X and Mathur, Sanjay (2021). Magnetic nanoparticle-based amplification of microRNA detection in body fluids for early disease diagnosis. J. Mat. Chem. B, 9 (1). S. 9 - 23. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 2050-7518

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Abstract

Circulating biomarkers such as microRNAs (miRNAs), short noncoding RNA strands, represent prognostic and diagnostic indicators for a variety of physiological disorders making their detection and quantification an attractive approach for minimally invasive early disease diagnosis. However, highly sensitive and selective detection methods are required given the generally low abundance of miRNAs in body fluids together with the presence of large amounts of other potentially interfering biomolecules. Although a variety of miRNA isolation and detection methods have been established in clinics, they usually require trained personnel and often constitute labor-, time- and cost-intensive approaches. During the last years, nanoparticle-based biosensors have received increasing attention due to their superior detection efficiency even in very low concentration regimes. This is based on their unique physicochemical properties in combination with their high surface area that allows for the immobilization of multiple recognition sites resulting in fast and effective recognition of analytes. Among various materials, magnetic nanoparticles have been identified as useful tools for the separation, concentration, and detection of miRNAs. Here, we review state-of-the-art technology with regard to magnetic particle-based miRNA detection from body fluids, critically discussing challenges and future perspective of such biosensors while comparing their handling, sensitivity as well as selectivity against the established miRNA isolation and detection methods.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gessner, Isabel UNSPECIFIED orcid.org/0000-0001-9674-161X UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brune, Veronika UNSPECIFIED orcid.org/0000-0001-5909-621X UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-567772
DOI:	10.1039/d0tb02165b
Journal or Publication Title:	J. Mat. Chem. B
Volume:	9
Number:	1
Page Range:	S. 9 - 23
Date:	2021
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	2050-7518
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DUPLEX-SPECIFIC NUCLEASE; QUALITY-CONTROL; RT-PCR; MIRNA; CANCER; BIOMARKERS; RNA; NANOTECHNOLOGY; EXPRESSION; STRATEGIES Multiple languages Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56777