Universität zu Köln

Stephan, Lisa ORCID: 0000-0002-1296-7548, Jakoby, Marc, Das, Arijit ORCID: 0000-0002-1108-6052, Koebke, Eva and Huelskamp, Martin (2021). Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies. PLoS One, 16 (5). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The directional movement and positioning of organelles and macromolecules is essential for regulating and maintaining cellular functions in eukaryotic cells. In plants, these processes are actin-based and driven by class XI myosins, which transport various cargos in a directed manner. As the analysis of myosin function is challenging due to high levels of redundancy, dominant negative acting truncated myosins have frequently been used to study intracellular transport processes. A comparison of the dominant negative effect of the coiled-coil domains and the GTD domains revealed a much stronger inhibition of P-body movement by the GTD domains. In addition, we show that the GTD domain does not inhibit P-body movement when driven by a hybrid myosin in which the GTD domain was replaced by DCP2. These data suggest that the dominant negative effect of myosin tails involves a competition of the GTD domains for cargo binding sites.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Stephan, Lisa UNSPECIFIED orcid.org/0000-0002-1296-7548 UNSPECIFIED Jakoby, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Das, Arijit UNSPECIFIED orcid.org/0000-0002-1108-6052 UNSPECIFIED Koebke, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-568273
DOI:	10.1371/journal.pone.0252327
Journal or Publication Title:	PLoS One
Volume:	16
Number:	5
Date:	2021
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ROOT HAIR; ARABIDOPSIS; ACTIN; MOVEMENT; GROWTH; GOLGI; CELLS; DIMERIZATION; RESISTANCE; MEMBRANE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56827