Universität zu Köln

Haiduk, Tiffany, Brockmann, Michael, Tillmann, Ramona-Liza, Pieper, Monika, Lusebrink, Jessica, Schildgen, Verena and Schildgen, Oliver ORCID: 0000-0003-4297-9627 (2021). Comparison of Biocartis IDYLLA(TM) cartridge assay with Qiagen GeneReader NGS for detection of targetable mutations in EGFR, KRAS/ NRAS, and BRAF genes. Exp. Mol. Pathol., 120. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1096-0945

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Abstract

Lung and colorectal cancers (CRC) have two of the highest mortality rates among all cancer types, and their occurrence and the need for personalized diagnostics and subsequent therapy were not influenced by the COVID19 pandemics. However, due to the disruption of established delivery chains, standard assays for in vitro diagnostics of those cancers were temporarily not available, forcing us to implement alternative testing methods that enabled at least basic therapy decision making. For this reason, we evaluated rapid testing on the Biocartis IdyllaTM platform (Biocartis, Mechelen, Belgium) for four important genes commonly mutated in lung and colorectal cancers, namely EGFR, NRAS, KRAS, and BRAF. Clinical specimens from which the mutation status has previously been determined using Next Generation Sequencing (NGS), were retested to determine whether IdyllaTM can offer accurate results. To compare the results, the sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) are calculated for each of the mutation types and then combined to determine the values of the IdyllaTM system in total, while setting NGS as the gold-standard basis the assays were compared with. Idylla testing thereby displayed acceptable sensitivity and specificity and delivered reliable results for initial therapy decisions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Haiduk, Tiffany UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Tillmann, Ramona-Liza UNSPECIFIED UNSPECIFIED UNSPECIFIED Pieper, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Lusebrink, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-569548
DOI:	10.1016/j.yexmp.2021.104634
Journal or Publication Title:	Exp. Mol. Pathol.
Volume:	120
Date:	2021
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1096-0945
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL LUNG-CANCER Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56954