Kerner, Konstantin, Nagano, Soshichiro, Lubbe, Annika and Hoecker, Ute ORCID: 0000-0002-5636-9777 (2021). Functional comparison of the WD-repeat domains of SPA1 and COP1 in suppression of photomorphogenesis. Plant Cell Environ., 44 (10). S. 3273 - 3283. HOBOKEN: WILEY. ISSN 1365-3040
Full text not available from this repository.Abstract
The Arabidopsis COP1/SPA complex acts as a cullin4-based E3 ubiquitin ligase to suppress photomorphogenesis in darkness. It is a tetrameric complex of two COP1 and two SPA proteins. Both COP1 and SPA are essential for the activity of this complex, and they both contain a C-terminal WD-repeat domain responsible for substrate recruitment and binding of DDB1. Here, we used a WD domain swap-approach to address the cooperativity of COP1 and SPA proteins. We found that expression of a chimeric COP1 carrying the WD-repeat domain of SPA1 mostly complemented the cop1-4-mutant phenotype in darkness, indicating that the WD repeat of SPA1 can replace the WD repeat of COP1. In the light, SPA1-WD partially substituted for COP1-WD. In contrast, expression of a chimeric SPA1 protein carrying the WD repeat of COP1 did not rescue the spa-mutant phenotype. Together, our findings demonstrate that a SPA1-type WD repeat is essential for COP1/SPA activity, while a COP1-type WD is in part dispensible. Moreover, a complex with four SPA1-WDs is more active than a complex with only two SPA1-WDs. A homology model of SPA1-WD based on the crystal structure of COP1-WD uncovered two insertions and several amino acid substitutions at the predicted substrate-binding pocket of SPA1-WD.
Item Type: | Journal Article | ||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-573166 | ||||||||||||||||||||
DOI: | 10.1111/pce.14148 | ||||||||||||||||||||
Journal or Publication Title: | Plant Cell Environ. | ||||||||||||||||||||
Volume: | 44 | ||||||||||||||||||||
Number: | 10 | ||||||||||||||||||||
Page Range: | S. 3273 - 3283 | ||||||||||||||||||||
Date: | 2021 | ||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||
ISSN: | 1365-3040 | ||||||||||||||||||||
Language: | English | ||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/57316 |
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