Universität zu Köln

Diaz-Jimenez, Maria, Wang, Mengying, Wang, Wanxue ORCID: 0000-0003-0606-6628, Isachenko, Evgenia, Rahimi, Gohar, Kumar, Pradeep, Mallmann, Peter, von Brandenstein, Melanie, Hidalgo, Manuel and Isachenko, Vladimir ORCID: 0000-0002-3674-543X (2022). Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature. Cell Tissue Banking, 23 (1). S. 17 - 30. DORDRECHT: SPRINGER. ISSN 1573-6814

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Abstract

Cryoprotectant-free vitrification is a common method for spermatozoa cryopreservation by direct plunging into liquid nitrogen. However, the commercial liquid nitrogen could be potentially contaminated by microorganisms. Warming temperature plays an essential role for quality of human spermatozoa after vitrification. This study aimed to evaluate comparatively a quality spermatozoa after vitrification in liquid nitrogen and clean liquid air as well as with two warming rates: at 42 degrees C and 45 degrees C. After performing of routine swim-up of normozoospermia samples, spermatozoa from the same ejaculate were divided into two groups: vitrified in liquid nitrogen (LN) and sterile liquid air (LA). Spermatozoa of LN group were warmed at 42 degrees C, and spermatozoa of LA groups were divided and warmed at 42 degrees C (LA42) and 45 degrees C (LA45). Then spermatozoa motility, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), reactive nitrogen species (RNS), and viability were assessed. It was no found significant differences in quality of spermatozoa from LN and LA groups in the motility, ROS, MMP, RNS rates after warming at 42 degrees C. A tendency to obtain better spermatozoa quality was found with using of warming by 42 degrees C in comparison with 45 degrees C. It was concluded that cryoprotectant-free vitrification by direct dropping of human spermatozoa into clean liquid air can be used as an alternative to cooling in liquid nitrogen. Warming of spermatozoa at 42 degrees C allows to preserve the spermatozoa physiological parameters.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diaz-Jimenez, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Mengying UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Wanxue UNSPECIFIED orcid.org/0000-0003-0606-6628 UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumar, Pradeep UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Hidalgo, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED orcid.org/0000-0002-3674-543X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-574175
DOI:	10.1007/s10561-021-09904-0
Journal or Publication Title:	Cell Tissue Banking
Volume:	23
Number:	1
Page Range:	S. 17 - 30
Date:	2022
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-6814
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OXIDATIVE STRESS; NITROSATIVE STRESS; DNA INTEGRITY; SPERM; CRYOPRESERVATION; SEMEN; MOTILITY; CONTAMINATION; QUALITY; EMBRYOS Multiple languages Cell Biology; Engineering, Biomedical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57417