Universität zu Köln

Peker, Esra, Erdogan, Alican J., Volkov, Alexander N. and Riemer, Jan (2021). Y Erv1 and Cytochrome c Mediate Rapid Electron Transfer via A Collision-Type Interaction. J. Mol. Biol., 433 (15). LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1089-8638

Full text not available from this repository.

Abstract

Being essential for oxidative protein folding in the mitochondrial intermembrane space, the mitochondrial disulfide relay relies on the electron transfer (ET) from the sulfhydryl oxidase Erv1 to cytochrome c (Cc). Using solution NMR spectroscopy, we demonstrate that while the yeast Cc-Erv1 system is functionally active, no observable binding of the protein partners takes place. The transient interaction between Erv1 and Cc can be rationalized by molecular modeling, suggesting that a large surface area of Erv1 can sustain a fast ET to Cc via a collision-type mechanism, without the need for a canonical protein complex formation. We suggest that, by preventing the direct ET to molecular oxygen (O-2), the collision-type Cc-Erv1 interaction plays a role in protecting the organism against reactive oxygen species. (C) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Peker, Esra UNSPECIFIED UNSPECIFIED UNSPECIFIED Erdogan, Alican J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Volkov, Alexander N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Riemer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-577882
DOI:	10.1016/j.jmb.2021.167045
Journal or Publication Title:	J. Mol. Biol.
Volume:	433
Number:	15
Date:	2021
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1089-8638
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DISULFIDE RELAY SYSTEM; MITOCHONDRIAL INTERMEMBRANE SPACE; NUCLEAR-MAGNETIC-RESONANCE; PROTEIN INTERACTIONS; SULFHYDRYL OXIDASE; ENCOUNTER COMPLEX; NMR-SPECTROSCOPY; IMPORT PATHWAY; MIA40; PLASTOCYANIN Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57788