Universität zu Köln

Zweerink, Susanne, Mesghenna, Senait, Mueck, Vera, Schulte, Sigrid, Kuetting, Fabian, Quaas, Alexander, Goeser, Tobias and Nierhoff, Dirk (2021). First evaluation of Neighbor of Punc E11 (NOPE) as a novel marker in human hepatocellular carcinoma. Cancer Biomark., 30 (1). S. 75 - 84. AMSTERDAM: IOS PRESS. ISSN 1875-8592

Full text not available from this repository.

Abstract

BACKGROUND: Hepatocellular carcinoma (HCC) is the second most common cause of cancer death worldwide and the search for clinically useful biomarkers is ongoing. Neighbor of Punc E11 (NOPE) is an established biomarker of murine HCC that remains undetectable in normal liver and at preneoplastic stages. OBJECTIVE: The aim of our study was to evaluate the presence of NOPE in human HCC. METHODS: Histologically confirmed HCC and corresponding non-tumor liver samples from 20 patients were analyzed for expression of NOPE using qRT-PCR and mRNA-in-situ technology in a conserved tissue context. RESULTS: In our cohort, 30% of HCC samples were expressing NOPE which proved particularly useful in non-cirrhotic HCC samples with up to 155-fold higher expression than in adult liver. Using mRNA-in-situ technology, NOPE was clearly identified within epithelial tumor cells of NOPE positive human HCCs. In our analyzed cohort, the combination of AFP with NOPE did not reach more than 40% sensitivity while GPC-3 and NOPE were complementary to each other reaching a combined sensitivity of 85.7%. CONCLUSIONS: This is the first characterization of NOPE as a potential biomarker for human HCC. Our results underline the value of NOPE as a complementing biomarker for human HCC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zweerink, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Mesghenna, Senait UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueck, Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulte, Sigrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuetting, Fabian UNSPECIFIED UNSPECIFIED UNSPECIFIED Quaas, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Goeser, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Nierhoff, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-578436
DOI:	10.3233/CBM-190819
Journal or Publication Title:	Cancer Biomark.
Volume:	30
Number:	1
Page Range:	S. 75 - 84
Date:	2021
Publisher:	IOS PRESS
Place of Publication:	AMSTERDAM
ISSN:	1875-8592
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLUTAMINE-SYNTHETASE; ALPHA-FETOPROTEIN; LIVER BIOPSIES; GLYPICAN-3; DIAGNOSIS; EXPRESSION; SERUM; BIOMARKERS; HSP70; TUMOR Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57843