Universität zu Köln

Liu, Yaobin, Mukherjee, Rukmini ORCID: 0000-0001-7955-6324, Bonn, Florian, Colby, Thomas, Matic, Ivan ORCID: 0000-0003-0170-7991, Glogger, Marius, Heilemann, Mike and Dikic, Ivan ORCID: 0000-0001-8156-9511 (2021). Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection. Cell Death Differ., 28 (10). S. 2957 - 2970. LONDON: SPRINGERNATURE. ISSN 1476-5403

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Abstract

SidE family of Legionella effectors catalyze non-canonical phosphoribosyl-linked ubiquitination (PR-ubiquitination) of host proteins during bacterial infection. SdeA localizes predominantly to ER and partially to the Golgi apparatus, and mediates serine ubiquitination of multiple ER and Golgi proteins. Here we show that SdeA causes disruption of Golgi integrity due to its ubiquitin ligase activity. The Golgi linking proteins GRASP55 and GRASP65 are PR-ubiquitinated on multiple serine residues, thus preventing their ability to cluster and form oligomeric structures. In addition, we found that the functional consequence of Golgi disruption is not linked to the recruitment of Golgi membranes to the growing Legionella-containing vacuoles. Instead, it affects the host secretory pathway. Taken together, our study sheds light on the Golgi manipulation strategy by which Legionella hijacks the secretory pathway and promotes bacterial infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Liu, Yaobin UNSPECIFIED UNSPECIFIED UNSPECIFIED Mukherjee, Rukmini UNSPECIFIED orcid.org/0000-0001-7955-6324 UNSPECIFIED Bonn, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Colby, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Matic, Ivan UNSPECIFIED orcid.org/0000-0003-0170-7991 UNSPECIFIED Glogger, Marius UNSPECIFIED UNSPECIFIED UNSPECIFIED Heilemann, Mike UNSPECIFIED UNSPECIFIED UNSPECIFIED Dikic, Ivan UNSPECIFIED orcid.org/0000-0001-8156-9511 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-579469
DOI:	10.1038/s41418-021-00830-y
Journal or Publication Title:	Cell Death Differ.
Volume:	28
Number:	10
Page Range:	S. 2957 - 2970
Date:	2021
Publisher:	SPRINGERNATURE
Place of Publication:	LONDON
ISSN:	1476-5403
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOPLASMIC-RETICULUM; PROTEIN TRAFFICKING; PNEUMOPHILA; DOMAINS; GRASP55 Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57946