Universität zu Köln

Guerrero-Castillo, Sergio ORCID: 0000-0002-6630-0562, van Strien, Joeri ORCID: 0000-0003-4285-4429, Brandt, Ulrich ORCID: 0000-0003-1869-6811 and Arnold, Susanne ORCID: 0000-0003-2667-5156 (2021). Ablation of mitochondrial DNA results in widespread remodeling of the mitochondrial complexome. Embo J., 40 (21). HOBOKEN: WILEY. ISSN 1460-2075

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Abstract

So-called rho 0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How cells adapt to survive ablation of oxidative phosphorylation remains poorly understood. Complexome profiling analysis of rho 0 cells covered 1,002 mitochondrial proteins and revealed changes in abundance and organization of numerous multiprotein complexes including previously not described assemblies. Beyond multiple subassemblies of complexes that would normally contain components encoded by mitochondrial DNA, we observed widespread reorganization of the complexome. This included distinct changes in the expression pattern of adenine nucleotide carrier isoforms, other mitochondrial transporters, and components of the protein import machinery. Remarkably, ablation of mitochondrial DNA hardly affected the complexes organizing cristae junctions indicating that the altered cristae morphology in rho 0 mitochondria predominantly resulted from the loss of complex V dimers required to impose narrow curvatures to the inner membrane. Our data provide a comprehensive resource for in-depth analysis of remodeling of the mitochondrial complexome in response to respiratory deficiency.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Guerrero-Castillo, Sergio UNSPECIFIED orcid.org/0000-0002-6630-0562 UNSPECIFIED van Strien, Joeri UNSPECIFIED orcid.org/0000-0003-4285-4429 UNSPECIFIED Brandt, Ulrich UNSPECIFIED orcid.org/0000-0003-1869-6811 UNSPECIFIED Arnold, Susanne UNSPECIFIED orcid.org/0000-0003-2667-5156 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582312
DOI:	10.15252/embj.2021108648
Journal or Publication Title:	Embo J.
Volume:	40
Number:	21
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1460-2075
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ATP SYNTHASE; SUCCINATE-DEHYDROGENASE; PROTEIN TRANSLOCASE; ACYLGLYCEROL KINASE; SENGERS SYNDROME; CONTACT SITE; SUBUNIT; CARRIER; IMPORT; COMPONENT Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58231