Universität zu Köln

Klupp, Fee, Kahlert, Christoph, Franz, Clemens, Halama, Niels, Schleussner, Nikolai, Wirsik, Naita M., Warth, Arne, Schmidt, Thomas ORCID: 0000-0002-7166-3675 and Ulrich, Alexis B. (2021). Granulin: An Invasive and Survival-Determining Marker in Colorectal Cancer Patients. Int. J. Mol. Sci., 22 (12). BASEL: MDPI. ISSN 1422-0067

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Abstract

Background: Granulin is a secreted, glycosylated peptide-originated by cleavage from a precursor protein-which is involved in cell growth, tumor invasion and angiogenesis. However, the specific prognostic impact of granulin in human colorectal cancer has only been studied to a limited extent. Thus, we wanted to assess the expression of granulin in colorectal cancer patients to evaluate its potential as a prognostic biomarker. Methods: Expressional differences of granulin in colorectal carcinoma tissue (n = 94) and corresponding healthy colon mucosa were assessed using qRT-PCR. Immunohistochemistry was performed in colorectal cancer specimens (n = 97), corresponding healthy mucosa (n = 47) and colorectal adenomas (n = 19). Subsequently, the results were correlated with histopathological and clinical patients' data. HCT-116 cells were transfected with siRNA for invasion and migration assays. Results: Immunohistochemistry and qRT-PCR revealed tumoral over expression of granulin in colorectal cancer specimens compared to corresponding healthy colon mucosa and adenomas. Tumoral overexpression of granulin was associated with a significantly impaired overall survival. Moreover, downregulation of granulin by siRNA significantly diminished the invasive capacities of HCT-116 cells in vitro. Conclusion: Expression of granulin differs in colorectal cancer tissue, adenomas and healthy colon mucosa. Furthermore, granulin features invasive and migrative capabilities and overexpression of granulin correlates with a dismal prognosis. This reveals its potential as a prognostic biomarker and granulin could be a worthwhile molecular target for individualized anticancer therapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klupp, Fee UNSPECIFIED UNSPECIFIED UNSPECIFIED Kahlert, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Franz, Clemens UNSPECIFIED UNSPECIFIED UNSPECIFIED Halama, Niels UNSPECIFIED UNSPECIFIED UNSPECIFIED Schleussner, Nikolai UNSPECIFIED UNSPECIFIED UNSPECIFIED Wirsik, Naita M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Warth, Arne UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Thomas UNSPECIFIED orcid.org/0000-0002-7166-3675 UNSPECIFIED Ulrich, Alexis B. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582340
DOI:	10.3390/ijms22126436
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	22
Number:	12
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPITHELIN PRECURSOR; GROWTH-FACTOR; HEPATOCELLULAR-CARCINOMA; PROGRANULIN; PROMOTES; CELLS; EXPRESSION; PROEPITHELIN; INFLAMMATION; CROSSROADS Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58234