Universität zu Köln

Fuchs, Alexander ORCID: 0000-0002-6543-5051, Gross, Sabine, Neumann, Tobias, Illerhauss, Anja, Vink, Hans, Klasen, Gerda, Gathof, Birgit and Annecke, Thorsten (2021). Immediate effects of whole blood donation on the endothelial surface layer and glycocalyx shedding. Blood Transf., 19 (3). S. 190 - 197. MILAN: SIMTIPRO SRL. ISSN 1723-2007

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Abstract

Background - The endothelium plays a pivotal role in regulating microvascular function, especially in situations associated with acute blood loss. Whether blood donation and the associated volume loss affects the dimensions of the endothelial surface layer (ESL) and glycocalyx integrity remains unknown. Materials and methods - This study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in healthy subjects shortly before and after a donation of 500 mL whole blood. A novel image acquisition and analysis software (GlycoCheckT) automatically calculated the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration into the ESL, in vessels between 5 and 25 mu m in diameter. Microvascular perfusion was measured by RBC filling percentage. Soluble glycocalyx components were determined in the peripheral circulation. Results - There was no significant immediate effect of whole blood donation on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change in PBR and change in RBC filling percentage (regression coefficient beta: -0.040; 95% confidence interval: -0.049 to -0.030; p<0.001). A significant reduction in plasma heparan sulphate (1,329 +/- 316 vs 1,237 +/- 275 ng/mL, p=0.005) and hyaluronan (94 +/- 18 vs 90 +/- 16 ng/mL, p=0.002) was noted, while syndecan-1 levels (30 [23-50] vs 29 [24-46] ng/mL, p=0.282) remained unchanged. Discussion - Dimensions and integrity of the ESL appear to remain stable following a 500 mL whole blood donation and reflect its ability to ensure microvascular function and perfusion.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fuchs, Alexander UNSPECIFIED orcid.org/0000-0002-6543-5051 UNSPECIFIED Gross, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Illerhauss, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Vink, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Klasen, Gerda UNSPECIFIED UNSPECIFIED UNSPECIFIED Gathof, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Annecke, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-585244
DOI:	10.2450/2020.0025-20
Journal or Publication Title:	Blood Transf.
Volume:	19
Number:	3
Page Range:	S. 190 - 197
Date:	2021
Publisher:	SIMTIPRO SRL
Place of Publication:	MILAN
ISSN:	1723-2007
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUBLINGUAL MICROVASCULAR GLYCOCALYX; HEMORRHAGIC-SHOCK; DYSFUNCTION Multiple languages Hematology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58524