Pradhan, Ashis Kumar, Kandasamy, Ganapathi, Chatterjee, Upasana, Bharadwaj, Anushree, Mathew, Sam J., Dohmen, R. Jurgen and Palanimurugan, R. (2021). Ribosome-associated quality control mediates degradation of the premature translation termination product Orf1p of ODC antizyme mRNA. FEBS Lett., 595 (15). S. 2015 - 2034. HOBOKEN: WILEY. ISSN 1873-3468

Full text not available from this repository.

Abstract

Decoding of OAZ1 (Ornithine decarboxylase AntiZyme 1) mRNA, which harbours two open reading frames (ORF1 and ORF2) interrupted by a naturally occurring Premature Termination Codon (PTC), produces an 8 kDa truncated polypeptide termed Orf1p, unless the PTC is bypassed by +1 ribosomal frameshifting. In this study, we identified Orf1p as an endogenous ubiquitin-dependent substrate of the 26S proteasome both in yeast and mammalian cells. Surprisingly, we found that the ribosome-associated quality control factor Rqc1 and the ubiquitin ligase Ltn1 are critical for Orf1p degradation. In addition, the cytosolic protein quality control chaperone system Hsp70/Hsp90 and their corresponding co-chaperones Sse1, Fes1, Sti1 and Cpr7 are also required for Orf1p proteolysis. Our study finds that Orf1p, which is naturally synthesized as a result of a premature translation termination event, requires the coordinated role of both ribosome-associated and cytosolic protein quality control factors for its degradation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Pradhan, Ashis KumarUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kandasamy, GanapathiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chatterjee, UpasanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bharadwaj, AnushreeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathew, Sam J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dohmen, R. JurgenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Palanimurugan, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-586642
DOI: 10.1002/1873-3468.14147
Journal or Publication Title: FEBS Lett.
Volume: 595
Number: 15
Page Range: S. 2015 - 2034
Date: 2021
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1873-3468
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
UBIQUITIN SYSTEM; COTRANSLATIONAL UBIQUITINATION; YEAST GENES; IN-VIVO; PROTEIN; PROTEASOME; PATHWAY; EXPRESSION; CELLS; HSP70Multiple languages
Biochemistry & Molecular Biology; Biophysics; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58664

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item