Universität zu Köln

Wewer, Vera ORCID: 0000-0002-0199-5228, Peisker, Helga, Gutbrod, Katharina, Al-Bahra, Mazen, Menche, Dirk, Amambo, Ngongeh Glory, Fombad, Fanny F., Njouendou, Abdel Jelil, Pfarr, Kenneth ORCID: 0000-0003-3096-2465, Wanji, Samuel, Hoerauf, Achim and Doermann, Peter (2021). Urine metabolites for the identification of Onchocerca volvulus infections in patients from Cameroon. Parasites Vectors, 14 (1). LONDON: BMC. ISSN 1756-3305

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Abstract

Background: The tropical disease onchocerciasis (river blindness), caused by Onchocerca volvulus filarial nematodes, is targeted for elimination by mass treatment with nematocidal and antimicrobial drugs. Diagnosis of O. volvulus infections is based on counts of skin-borne microfilariae, but additional diagnostic tools, e.g. worm- or host-derived small RNAs, proteins or metabolites, are required for high-throughput screening. N-acetyltyramine-O,beta-glucuronide (NATOG) was suggested as a biomarker for onchocerciasis but its viability as diagnostic tool has been challenged. Methods: We performed a screening program of urine samples from individuals from Cameroon infected with O. volvulus, Loa loa, Mansonella perstans or a combination thereof. Urine metabolites were measured by liquid chromatography-mass spectrometry (LC-MS). Principle component analysis (PCA) revealed that onchocerciasis causes complex changes of the urine metabolome. Results: The mean NATOG content was elevated in urine of O. volvulus-infected compared with non-infected individuals, but NATOG levels showed considerable variation. However, 13.8% of all O. volvulus-infected individuals had high NATOG levels never reached by individuals without filarial infections or only infected with L. loa or M. perstans. Therefore, the identification of individuals with high NATOG levels might be used to screen for the elimination of onchocerciasis after mass drug application. Additional metabolites, including a compound identified as cinnamoylglycine, had high PC1/PC2 loadings in the data set. Mean levels of cinnamoylglycine were increased in O. volvulus-infected individuals, and 17.2% of all O. volvulus individuals had elevated cinnamoylglycine levels not reached by the controls. Conclusions: On an individual level, NATOG alone had poor discriminative power distinguishing infected from non-infected individuals. However, 13.8% of all O. volvulus-infected individuals had NATOG levels never reached by individuals without filarial infections or infected with only L. loa or M. perstans. Discrimination of O. volvulus infections from controls or individuals suffering from multiple infections was improved by the measurement of additional metabolites, e.g. cinnamoylglycine. Thus, measuring a combination of urine metabolites may provide a way to assess onchocerciasis on the population level. This provides the possibility to design a strategy for large-scale onchocerciasis epidemiological screening programs based on urine rather than invasive techniques.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wewer, Vera UNSPECIFIED orcid.org/0000-0002-0199-5228 UNSPECIFIED Peisker, Helga UNSPECIFIED UNSPECIFIED UNSPECIFIED Gutbrod, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Al-Bahra, Mazen UNSPECIFIED UNSPECIFIED UNSPECIFIED Menche, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Amambo, Ngongeh Glory UNSPECIFIED UNSPECIFIED UNSPECIFIED Fombad, Fanny F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Njouendou, Abdel Jelil UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfarr, Kenneth UNSPECIFIED orcid.org/0000-0003-3096-2465 UNSPECIFIED Wanji, Samuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoerauf, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-587765
DOI:	10.1186/s13071-021-04893-1
Journal or Publication Title:	Parasites Vectors
Volume:	14
Number:	1
Date:	2021
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1756-3305
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BIOMARKER; ALPHA Multiple languages Parasitology; Tropical Medicine Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58776