Universität zu Köln

Iwama, Taiga, Kano, Kuniyuki ORCID: 0000-0002-4539-5750, Saigusa, Daisuke, Ekroos, Kim, van Echten-Deckert, Gerhild, Vogt, Johannes ORCID: 0000-0003-2439-8247 and Aoki, Junken (2021). Development of an On-Tissue Derivatization Method for MALDI Mass Spectrometry Imaging of Bioactive Lipids Containing Phosphate Monoester Using Phos-tag. Anal. Chem., 93 (8). S. 3867 - 3876. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-6882

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Abstract

Matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is an emerging label-free method for mapping the distribution of diverse molecular species in tissue sections. Despite recent progress in MALDI-MSI analyses of lipids, it is still difficult to visualize minor bioactive lipids including lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P). Here, we have developed a novel on-tissue derivatization method using Phos-tag, a zinc complex that specifically binds to a phosphate monoester group. MALDI-MSI with Phos-tag derivatization made it possible to image LPA and S1P in the murine brain. Furthermore, we were able to visualize other low-abundance lipids containing phosphate monoester, such as phosphatidic acid and ceramide-1-phosphate. Compared with conventional MALDI-MS, this derivatization produced LPA images with high spatial accuracy discriminating LPA artificially produced during MALDI-MS analysis. In mice with deficiencies in enzymes that degrade LPA and S1P, we observed marked S1P and/or LPA accumulation in specific regions of the brain. Thus, the present study provides a simple and optimal way to reveal the spatial localization of potent bioactive lipid phosphates such as LPA and S1P in tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Iwama, Taiga UNSPECIFIED UNSPECIFIED UNSPECIFIED Kano, Kuniyuki UNSPECIFIED orcid.org/0000-0002-4539-5750 UNSPECIFIED Saigusa, Daisuke UNSPECIFIED UNSPECIFIED UNSPECIFIED Ekroos, Kim UNSPECIFIED UNSPECIFIED UNSPECIFIED van Echten-Deckert, Gerhild UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogt, Johannes UNSPECIFIED orcid.org/0000-0003-2439-8247 UNSPECIFIED Aoki, Junken UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-589770
DOI:	10.1021/acs.analchem.0c04479
Journal or Publication Title:	Anal. Chem.
Volume:	93
Number:	8
Page Range:	S. 3867 - 3876
Date:	2021
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1520-6882
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58977