Malinova, Tsveta S., Angulo-Urarte, Ana ORCID: 0000-0002-8024-814X, Nuechel, Julian, Tauber, Marina, van der Stoel, Miesje M., Janssen, Vera ORCID: 0000-0002-0788-8060, de Haan, Annett, Groenen, Anouk G., Tebbens, Merel ORCID: 0000-0002-9044-9068, Graupera, Mariona, Plomann, Markus and Huveneers, Stephan ORCID: 0000-0002-1091-475X (2021). A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis. Nat. Commun., 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2041-1723

Full text not available from this repository.

Abstract

Angiogenic sprouting relies on collective migration and coordinated rearrangements of endothelial leader and follower cells. VE-cadherin-based adherens junctions have emerged as key cell-cell contacts that transmit forces between cells and trigger signals during collective cell migration in angiogenesis. However, the underlying molecular mechanisms that govern these processes and their functional importance for vascular development still remain unknown. We previously showed that the F-BAR protein PACSIN2 is recruited to tensile asymmetric adherens junctions between leader and follower cells. Here we report that PACSIN2 mediates the formation of endothelial sprouts during angiogenesis by coordinating collective migration. We show that PACSIN2 recruits the trafficking regulators EHD4 and MICAL-L1 to the rear end of asymmetric adherens junctions to form a recycling endosome-like tubular structure. The junctional PACSIN2/EHD4/MICAL-L1 complex controls local VE-cadherin trafficking and thereby coordinates polarized endothelial migration and angiogenesis. Our findings reveal a molecular event at force-dependent asymmetric adherens junctions that occurs during the tug-of-war between endothelial leader and follower cells, and allows for junction-based guidance during collective migration in angiogenesis. Communication between endothelial leader and follower cells during collective cell migration is crucial for vascular development. Here, the authors show that PACSIN2 guides collective cell migration and angiogenesis by recruiting a protein trafficking complex to asymmetric cell-cell junctions, controlling local junction plasticity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Malinova, Tsveta S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Angulo-Urarte, AnaUNSPECIFIEDorcid.org/0000-0002-8024-814XUNSPECIFIED
Nuechel, JulianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tauber, MarinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Stoel, Miesje M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Janssen, VeraUNSPECIFIEDorcid.org/0000-0002-0788-8060UNSPECIFIED
de Haan, AnnettUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Groenen, Anouk G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tebbens, MerelUNSPECIFIEDorcid.org/0000-0002-9044-9068UNSPECIFIED
Graupera, MarionaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Plomann, MarkusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huveneers, StephanUNSPECIFIEDorcid.org/0000-0002-1091-475XUNSPECIFIED
URN: urn:nbn:de:hbz:38-590288
DOI: 10.1038/s41467-021-22873-y
Journal or Publication Title: Nat. Commun.
Volume: 12
Number: 1
Date: 2021
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ADHERENS JUNCTIONS; CELL-MIGRATION; ALPHA-CATENIN; MECHANOTRANSDUCTION; BINDING; PROTEIN; REARRANGEMENTS; STABILIZATION; STABILITY; TURNOVERMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59028

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item