Ishikawa, Yuuma, Cassan, Cedric, Kadeer, Aikeranmu, Yuasa, Koki, Sato, Nozomu, Sonoike, Kintake ORCID: 0000-0002-5327-473X, Kaneko, Yasuko, Miyagi, Atsuko ORCID: 0000-0003-4314-6555, Takahashi, Hiroko, Ishikawa, Toshiki, Yamaguchi, Masatoshi ORCID: 0000-0002-1396-5376, Nishiyama, Yoshitaka, Hihara, Yukako, Gibon, Yves ORCID: 0000-0001-8161-1089 and Kawai-Yamada, Maki (2021). The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803. Plant Cell Physiol., 62 (4). S. 668 - 678. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

NADP(+), the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD(+) to NADP(+). However, the physiological role of phosphorylation of NAD(+ )to NADP(+) in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme that NAD(+) accumulation in slr0400-deficient mutant results in the unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the Photosystem II (PSII) photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain the photosynthetic capacity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ishikawa, YuumaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cassan, CedricUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kadeer, AikeranmuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yuasa, KokiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sato, NozomuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sonoike, KintakeUNSPECIFIEDorcid.org/0000-0002-5327-473XUNSPECIFIED
Kaneko, YasukoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Miyagi, AtsukoUNSPECIFIEDorcid.org/0000-0003-4314-6555UNSPECIFIED
Takahashi, HirokoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ishikawa, ToshikiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yamaguchi, MasatoshiUNSPECIFIEDorcid.org/0000-0002-1396-5376UNSPECIFIED
Nishiyama, YoshitakaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hihara, YukakoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gibon, YvesUNSPECIFIEDorcid.org/0000-0001-8161-1089UNSPECIFIED
Kawai-Yamada, MakiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-594045
DOI: 10.1093/pcp/pcab023
Journal or Publication Title: Plant Cell Physiol.
Volume: 62
Number: 4
Page Range: S. 668 - 678
Date: 2021
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1471-9053
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HETEROTROPHIC GROWTH; ARABIDOPSIS; ENZYME; SUBUNITMultiple languages
Plant Sciences; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59404

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