Zimmermann, Sandra E., Benstein, Ruben M., Flores-Tornero, Maria ORCID: 0000-0002-9296-0070, Blau, Samira, Anoman, Armand D., Rosa-Tellez, Sara, Gerlich, Silke C., Salem, Mohamed A., Alseekh, Saleh ORCID: 0000-0003-2067-5235, Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Wewer, Vera ORCID: 0000-0002-0199-5228, Fluegge, Ulf-Ingo, Jacoby, Richard P., Fernie, Alisdair R., Giavalisco, Patrick, Ros, Roc and Krueger, Stephan (2021). The phosphorylated pathway of serine biosynthesis links plant growth with nitrogen metabolism. Plant Physiol., 186 (3). S. 1487 - 1507. CARY: OXFORD UNIV PRESS INC. ISSN 1532-2548

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Abstract

Because it is the precursor for various essential cellular components, the amino acid serine is indispensable for every living organism. In plants, serine is synthesized by two major pathways: photorespiration and the phosphorylated pathway of serine biosynthesis (PPSB). However, the importance of these pathways in providing serine for plant development is not fully understood. In this study, we examine the relative contributions of photorespiration and PPSB to providing serine for growth and metabolism in the C3 model plant Arabidopsis thaliana. Our analyses of cell proliferation and elongation reveal that PPSB-derived serine is indispensable for plant growth and its loss cannot be compensated by photorespiratory serine biosynthesis. Using isotope labeling, we show that PPSB-deficiency impairs the synthesis of proteins and purine nucleotides in plants. Furthermore, deficiency in PPSB-mediated serine biosynthesis leads to a strong accumulation of metabolites related to nitrogen metabolism. This result corroborates N-15-isotope labeling in which we observed an increased enrichment in labeled amino acids in PPSB-deficient plants. Expression studies indicate that elevated ammonium uptake and higher glutamine synthetase/glutamine oxoglutarate aminotransferase (GS/GOGAT) activity causes this phenotype. Metabolic analyses further show that elevated nitrogen assimilation and reduced amino acid turnover into proteins and nucleotides are the most likely driving forces for changes in respiratory metabolism and amino acid catabolism in PPSB-deficient plants. Accordingly, we conclude that even though photorespiration generates high amounts of serine in plants, PPSB-derived serine is more important for plant growth and its deficiency triggers the induction of nitrogen assimilation, most likely as an amino acid starvation response.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Zimmermann, Sandra E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Benstein, Ruben M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Flores-Tornero, MariaUNSPECIFIEDorcid.org/0000-0002-9296-0070UNSPECIFIED
Blau, SamiraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Anoman, Armand D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rosa-Tellez, SaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gerlich, Silke C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salem, Mohamed A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Alseekh, SalehUNSPECIFIEDorcid.org/0000-0003-2067-5235UNSPECIFIED
Kopriva, StanislavUNSPECIFIEDorcid.org/0000-0002-7416-6551UNSPECIFIED
Wewer, VeraUNSPECIFIEDorcid.org/0000-0002-0199-5228UNSPECIFIED
Fluegge, Ulf-IngoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jacoby, Richard P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fernie, Alisdair R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Giavalisco, PatrickUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ros, RocUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-594512
DOI: 10.1093/plphys/kiab167
Journal or Publication Title: Plant Physiol.
Volume: 186
Number: 3
Page Range: S. 1487 - 1507
Date: 2021
Publisher: OXFORD UNIV PRESS INC
Place of Publication: CARY
ISSN: 1532-2548
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GLUTAMINE-SYNTHETASE GLN1/2; ONE-CARBON METABOLISM; ARABIDOPSIS-THALIANA; GAS-CHROMATOGRAPHY; DEHYDROGENASE; AMMONIUM; ACID; 3-PHOSPHOGLYCERATE; EXPRESSION; GLYCINEMultiple languages
Plant SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59451

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