Universität zu Köln

Chen, Sharon C. -A., Halliday, Catriona L., Hoenigl, Martin ORCID: 0000-0002-1653-2824, Cornely, Oliver A. and Meyer, Wieland ORCID: 0000-0001-9933-8340 (2021). Scedosporium and Lomentospora Infections: Contemporary Microbiological Tools for the Diagnosis of Invasive Disease. J. Fungi, 7 (1). BASEL: MDPI. ISSN 2309-608X

Full text not available from this repository.

Abstract

Scedosporium/Lomentospora fungi are increasingly recognized pathogens. As these fungi are resistant to many antifungal agents, early diagnosis is essential for initiating targeted drug therapy. Here, we review the microbiological tools for the detection and diagnosis of invasive scedosporiosis and lomentosporiosis. Of over 10 species, Lomentospora prolificans, Scedosporium apiospermum, S. boydii and S. aurantiacum cause the majority of infections. Definitive diagnosis relies on one or more of visualization, isolation or detection of the fungus from clinical specimens by microscopy techniques, culture and molecular methods such as panfungal PCR or genus-/species-specific multiplex PCR. For isolation from respiratory tract specimens, selective media have shown improved isolation rates. Species identification is achieved by macroscopic and microscopic examination of colonies, but species should be confirmed by ITS with or without beta-tubulin gene sequencing or other molecular methods. Matrix-assisted laser desorption ionization-time of flight mass spectrometry databases are improving but may need supplementation by in-house spectra for species identification. Reference broth microdilution methods is preferred for antifungal susceptibility testing. Next-generation sequencing technologies have good potential for characterization of these pathogens. Diagnosis of Scedosporium/Lomentospora infections relies on multiple approaches encompassing both phenotypic- and molecular-based methods.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chen, Sharon C. -A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Halliday, Catriona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoenigl, Martin UNSPECIFIED orcid.org/0000-0002-1653-2824 UNSPECIFIED Cornely, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Wieland UNSPECIFIED orcid.org/0000-0001-9933-8340 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-595890
DOI:	10.3390/jof7010023
Journal or Publication Title:	J. Fungi
Volume:	7
Number:	1
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; PSEUDALLESCHERIA-BOYDII; FUNGAL-INFECTIONS; CYSTIC-FIBROSIS; PCR ASSAY; IDENTIFICATION; PROLIFICANS; APIOSPERMUM; ASPERGILLUS Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59589