Universität zu Köln

Sattler, Janko ORCID: 0000-0003-1415-4169, Noster, Janina ORCID: 0000-0002-8802-6605, Brunke, Anne, Plum, Georg, Wiegel, Pia, Kurzai, Oliver ORCID: 0000-0002-7277-2646, Meis, Jacques F. and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2021). Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris. J. Fungi, 7 (2). BASEL: MDPI. ISSN 2309-608X

Full text not available from this repository.

Abstract

Candida auris is an emerging pathogen with resistance to many commonly used antifungal agents. Infections with C. auris require rapid and reliable detection methods to initiate successful medical treatment and contain hospital outbreaks. Conventional identification methods are prone to errors and can lead to misidentifications. PCR-based assays, in turn, can provide reliable results with low turnaround times. However, only limited data are available on the performance of commercially available assays for C. auris detection. In the present study, the two commercially available PCR assays AurisID (OLM, Newcastle Upon Tyne, UK) and Fungiplex Candida Auris RUO Real-Time PCR (Bruker, Bremen, Germany) were challenged with 29 C. auris isolates from all five clades and eight other Candida species as controls. AurisID reliably detected C. auris with a limit of detection (LoD) of 1 genome copies/reaction. However, false positive results were obtained with high DNA amounts of the closely related species C. haemulonii, C. duobushaemulonii and C. pseudohaemulonii. The Fungiplex Candida Auris RUO Real-Time PCR kit detected C. auris with an LoD of 9 copies/reaction. No false positive results were obtained with this assay. In addition, C. auris could also be detected in human blood samples spiked with pure fungal cultures by both kits. In summary, both kits could detect C. auris-DNA at low DNA concentrations but differed slightly in their limits of detection and specificity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sattler, Janko UNSPECIFIED orcid.org/0000-0003-1415-4169 UNSPECIFIED Noster, Janina UNSPECIFIED orcid.org/0000-0002-8802-6605 UNSPECIFIED Brunke, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Plum, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiegel, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurzai, Oliver UNSPECIFIED orcid.org/0000-0002-7277-2646 UNSPECIFIED Meis, Jacques F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-596393
DOI:	10.3390/jof7020154
Journal or Publication Title:	J. Fungi
Volume:	7
Number:	2
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59639