Shein, Aye Mya Sithu, Wannigama, Dhammika Leshan ORCID: 0000-0001-6316-1221, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Hurst, Cameron, Abe, Shuichi, Hongsing, Parichart, Chantaravisoot, Naphat, Saethang, Thammakorn, Luk-in, Sirirat, Liao, Tingting, Nilgate, Sumanee, Rirerm, Ubolrat, Kueakulpattana, Naris, Laowansiri, Matchima, Srisakul, Sukrit, Muhummudaree, Netchanok, Techawiwattanaboon, Teerasit, Gan, Lin, Xu, Chenchen, Kupwiwat, Rosalyn, Phattharapornjaroen, Phatthranit, Rojanathanes, Rojrit, Leelahavanichkul, Asada and Chatsuwan, Tanittha (2021). Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections. Sci Rep, 11 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

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Abstract

Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae, a common pathogen causing catheter-related biofilm infections in vascular catheters, has become a serious therapeutic challenge that must be addressed urgently. Although colistin and EDTA have successful roles for eradicating biofilms, no in vitro and in vivo studies have investigated their efficacy in catheter-related biofilm infections of colistin-resistant K. pneumoniae. In this study, colistin resistance was significantly reversed in both planktonic and mature biofilms of colistin-resistant K. pneumoniae by a combination of colistin (0.25-1 mu g/ml) with EDTA (12 mg/ml). This novel colistin-EDTA combination was also demonstrated to have potent efficacy in eradicating colistin-resistant K. pneumoniae catheter-related biofilm infections, and eliminating the risk of recurrence in vivo. Furthermore, this study revealed significant therapeutic efficacy of colistin-EDTA combination in reducing bacterial load in internal organs, lowering serum creatinine, and protecting treated mice from mortality. Altered in vivo expression of different virulence genes indicate bacterial adaptive responses to survive in hostile environments under different treatments. According to these data discovered in this study, a novel colistin-EDTA combination provides favorable efficacy and safety for successful eradication of colistin-resistant K. pneumonia catheter-related biofilm infections.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Shein, Aye Mya SithuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wannigama, Dhammika LeshanUNSPECIFIEDorcid.org/0000-0001-6316-1221UNSPECIFIED
Higgins, Paul G.UNSPECIFIEDorcid.org/0000-0001-8677-9454UNSPECIFIED
Hurst, CameronUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Abe, ShuichiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hongsing, ParichartUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chantaravisoot, NaphatUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saethang, ThammakornUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Luk-in, SiriratUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liao, TingtingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nilgate, SumaneeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rirerm, UbolratUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kueakulpattana, NarisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Laowansiri, MatchimaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Srisakul, SukritUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Muhummudaree, NetchanokUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Techawiwattanaboon, TeerasitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gan, LinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xu, ChenchenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kupwiwat, RosalynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Phattharapornjaroen, PhatthranitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rojanathanes, RojritUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leelahavanichkul, AsadaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chatsuwan, TanitthaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-602762
DOI: 10.1038/s41598-021-01052-5
Journal or Publication Title: Sci Rep
Volume: 11
Number: 1
Date: 2021
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PSEUDOMONAS-AERUGINOSA; ESCHERICHIA-COLI; LOCK SOLUTION; CALCIUM-EDTA; GENTAMICIN; VANCOMYCIN; DIAGNOSIS; SPECTRUM; THERAPY; UPDATEMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/60276

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