Universität zu Köln

Koeditz, Barbara, von Brandenstein, Melanie, Huerta-Arana, Manuel and Fries, Jochen W. U. (2022). Novel noninvasive marker of regression of clear cell renal cell carcinoma (ccRCC) (Publication with Expression of Concern. See vol. 48, pg. 392, 2022). Turk. J. Urol., 48 (1). S. 49 - 58. SISLI: AVES. ISSN 2149-3057

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Abstract

Objective: Analyzing protein kinase C (PKC) alpha, iota, and zeta as well as levels of Mxi-2 and Vim3 in regressive clear cell renal carcinomas (ccRCCs) and urine samples. Material and methods: Fresh samples of ccRCCs (predominantly pT1a/b) with different degrees of regression (<10%, 30%, 50%, and >= 70%) vs normal renal tissue and oncocytomas were studied by Western blot, using antibodies of different PKC isoforms. Urine samples from these tumors were analyzed by ELISA (PKC isoforms, Mxi-2, and Vim3). Results: With increasing degree of regression beyond 10%, nuclear Mxi-2 and Vim3 were highly overexpressed in fresh tumor samples. In urine samples, Vim3 was significantly overexpressed in oncocytoma and downregulated in RCCs with 70% regression. Western blot analysis shows that PKC alpha and iota levels were significantly increased in fresh tumor tissue samples (tumors with >= 30% regression). PKC zeta was expressed in normal kidney and significantly increased in oncocytoma but not found in ccRCCs. In patients' urines, Mxi-2 was significantly reduced (regression > 50%), while PKC isofonn alpha was significantly increased by advanced regression rate. PKC iota in patients' urine was overexpressed in oncocytoma and reduced in all ccRCC urines. Conclusion: Tumor regression in ccRCC tissue shows strong nuclear overexpression of Mxi-2, Vim3, and PKC alpha and iota. In respective urines, PKC alpha was overexpressed; PKC iota was decreased. Mxi-2 and Vim3 decreased with increasing regression rates. These reagents could serve as noninvasive ccRCC markers for regression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koeditz, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Huerta-Arana, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-657503
DOI:	10.5152/tud.2022.21259
Journal or Publication Title:	Turk. J. Urol.
Volume:	48
Number:	1
Page Range:	S. 49 - 58
Date:	2022
Publisher:	AVES
Place of Publication:	SISLI
ISSN:	2149-3057
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-KINASE-C; EXPRESSION; ETA Multiple languages Urology & Nephrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/65750