Universität zu Köln

Wang, Wanxue ORCID: 0000-0003-0606-6628, Pei, Cheng ORCID: 0000-0003-0556-3972, Isachenko, Evgenia, Zhou, Yang, Wang, Mengying, Rahimi, Gohar, Liu, Wensheng, Mallmann, Peter and Isachenko, Vladimir ORCID: 0000-0002-3674-543X (2022). Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor. Int. J. Mol. Sci., 23 (20). BASEL: MDPI. ISSN 1422-0067

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Abstract

Introduction: The in vitro culture of primordial follicles is the only available option for preserving fertility in prepubertal girls with malignant tumors. The cultivation of primordial follicles in scaffolds as artificial ovaries is a promising approach for this. Methods: Dissociated follicles were placed into an artificial ovarian scaffold composed of fibrinogen and thrombin. The follicles were cultured in a dish dedicated to live cell imaging and observed for growth using immunofluorescence and development via optical microscopy. The morphology of the follicles in the scaffold was three-dimensionally reconstructed using the Imaris software. Growth and development were also quantified. Results: The morphology of artificial ovaries began to degrade over time. Within approximately 7 days, primordial follicles were activated and grew into secondary follicles. A comparison of optical and confocal microscopy results revealed the superior detection of live cells using confocal microscopy. The three-dimensional reconstruction of the confocal microscopy data enabled the automatic enumeration and evaluation of the overall morphology of many follicles. Conclusions: The novel artificial ovary-enabled primordial follicles to enter the growth cycle after activation and grow into secondary follicles. The use of a fibrin scaffold as a carrier preserves the developmental potential of primordial germ cells and is a potentially effective method for preserving fertility in prepubertal girls.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Wanxue UNSPECIFIED orcid.org/0000-0003-0606-6628 UNSPECIFIED Pei, Cheng UNSPECIFIED orcid.org/0000-0003-0556-3972 UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhou, Yang UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Mengying UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Wensheng UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED orcid.org/0000-0002-3674-543X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-662199
DOI:	10.3390/ijms232012419
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	23
Number:	20
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FOLLICULAR DEVELOPMENT; ANTRAL FOLLICLES; TISSUE; TRANSPLANTATION; GROWTH; STAGE; MICE Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66219