Universität zu Köln

Renner, Alexander M. ORCID: 0000-0002-8267-6690, Derichsweiler, Christina, Ilyas, Shaista, Gessner, Isabel ORCID: 0000-0001-9674-161X, Fries, Jochen W. U. and Mathur, Sanjay (2022). High efficiency capture of biomarker miRNA15a for noninvasive diagnosis of malignant kidney tumors. Biomater. Sci., 10 (4). S. 1113 - 1123. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 2047-4849

Full text not available from this repository.

Abstract

To date, there are no preoperative and quantitative dynamics in clinical practice that can reliably differentiate between a benign and malignant renal cell carcinoma (RCC). For monitoring different analytes in body fluids, more than 40 different molecular biomarkers have been identified, however, they are associated with limited clinical sensitivity and/or non-optimal specificity due to their leaky nature. Previous work on RCC demonstrated the miRNA15a to be reliable and novel biomarker with 98.1% specificity and 100% sensitivity. Despite the high potential of miRNA15a biomarker, its clinical application is considerably hampered by the insensitive nature of the detection methods and low concentration of biomarker in samples that is aggravated by the high level of contamination due to other solutes present in body fluids. In this work, a non-invasive quantitative approach is demonstrated to overcome such diagnostics issues through biotin-streptavidin binding and fluorescence active magnetic nanocarriers that ensured prompt isolation, enrichment and purification of the biomarker miRNA15a from urine. The study demonstrates that detectable low levels of these miRNAs through miRNA capturing nanocarriers can potentially function as advanced diagnostic markers for the non-invasive investigation and early detection of renal cancer.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Renner, Alexander M. UNSPECIFIED orcid.org/0000-0002-8267-6690 UNSPECIFIED Derichsweiler, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Ilyas, Shaista UNSPECIFIED UNSPECIFIED UNSPECIFIED Gessner, Isabel UNSPECIFIED orcid.org/0000-0001-9674-161X UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-663403
DOI:	10.1039/d1bm01737c
Journal or Publication Title:	Biomater. Sci.
Volume:	10
Number:	4
Page Range:	S. 1113 - 1123
Date:	2022
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	2047-4849
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICRORNA 15A; CANCER; STREPTAVIDIN; EXPRESSION; MIR-210; BIOPSY; ALPHA Multiple languages Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66340